秋水仙酰胺在海胆卵中的作用。
Action of colcemid in sea urchin eggs.
作者信息
Zimmerman A M, Zimmerman S
出版信息
J Cell Biol. 1967 Aug;34(2):483-8. doi: 10.1083/jcb.34.2.483.
The effects of Colcemid, the deacetyl-N-methyl derivative of colchicine, on the eggs of Arbacia punctulata were investigated. Colcemid in concentrations of 2.7 x 10(-5)M or greater blocks syngamy (the fusion of the pronuclei) in these eggs. Although a tenfold decrease in concentration of Colcemid usually permits the pronuclei to fuse, the subsequent division is blocked. In the sea urchin egg, the duration of presyngamy is about 15 min during which time there is no DNA synthesis. However, DNA synthesis is recorded in Colcemid-blocked cells prior to syngamy. Radioautographs of Colcemid-blocked cells which were immersed into thymidine-(3)H exhibited silver grains above each of the pronuclei. The action of Colcemid on Arbacia eggs is reversible. Nevertheless, exposures to 2.7 x 10(-5)M Colcemid for only 3 min, initiated 5 min after insemination, caused delays of 70 min in subsequent division. In general, cells are more sensitive to Colcemid prior to the time when the mitotic spindle is being assembled than at presyngamy stages. The results are discussed in terms of Colcemid action on pronuclear fusion and cell division.
研究了秋水仙碱的脱乙酰-N-甲基衍生物秋水仙酰胺对刺冠海胆卵的影响。浓度为2.7×10⁻⁵M或更高的秋水仙酰胺会阻断这些卵中的配子融合（原核融合）。虽然秋水仙酰胺浓度降低十倍通常会使原核融合，但随后的分裂会被阻断。在海胆卵中，配子融合前的持续时间约为15分钟，在此期间没有DNA合成。然而，在配子融合前，秋水仙酰胺阻断的细胞中记录到了DNA合成。将浸入胸腺嘧啶核苷-³H中的秋水仙酰胺阻断细胞制成放射自显影片，在每个原核上方都显示出银颗粒。秋水仙酰胺对刺冠海胆卵的作用是可逆的。然而，在受精后5分钟开始仅暴露于2.7×10⁻⁵M秋水仙酰胺3分钟，会导致随后的分裂延迟70分钟。一般来说，在有丝分裂纺锤体组装时，细胞对秋水仙酰胺比在配子融合前期更敏感。根据秋水仙酰胺对原核融合和细胞分裂的作用对结果进行了讨论。
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