Surger Maximilian, Angelov Angel, Liebl Wolfgang
Chair of Microbiology, Technical University of Munich, Emil-Ramann-Str. 4, 85354 Freising-Weihenstephan, Germany.
Biotechnol Biofuels. 2020 Apr 15;13:70. doi: 10.1186/s13068-020-01706-y. eCollection 2020.
The natural production of olefins (unsaturated aliphatic hydrocarbons) by certain bacterial genera represents an alternative and sustainable source of biofuels and lubricant components. The biochemical steps of olefin biosynthesis via the ole pathway encoded by have been unraveled recently, and the occurrence of olefins has been reported for several Gram-negative and Gram-positive bacteria. However, the distribution and diversity of olefins among the Gram-positive bacteria has not been studied in detail.
We report the distribution of olefin synthesis gene clusters in the bacterial domain and focus on the olefin composition and the determinants of olefin production within the phylum of . The olefin profiles of numerous genera of the order were analyzed by GC/MS. We describe for the first time olefin synthesis in representatives of the genera , , , , , , , , and . By exchange of the native genes of with the corresponding genes of actinobacteria producing different olefins, we demonstrate that the olefin composition can be manipulated with respect to chain length and isomer composition.
This study provides a catalogue of the diversity of olefin structures found in the Our gene swapping data indicate that the olefin structures are fundamentally determined by the substrate specificity of OleA, and at the same time by the availability of a sufficient supply of suitable fatty acyl-CoA substrates from cellular fatty acid metabolism. This makes OleA of Gram-positive bacteria a promising target for structural analysis and protein engineering aiming to generate olefin chain lengths and isomer profiles which are designed to match the requirements of various industrial applications.
某些细菌属天然产生烯烃（不饱和脂肪烃），这代表了生物燃料和润滑剂成分的一种替代且可持续的来源。最近，通过由[具体基因]编码的烯烃途径进行烯烃生物合成的生化步骤已被阐明，并且已经报道了几种革兰氏阴性菌和革兰氏阳性菌中存在烯烃。然而，革兰氏阳性菌中烯烃的分布和多样性尚未得到详细研究。
我们报告了细菌域中烯烃合成基因簇的分布，并重点关注[具体菌门]内的烯烃组成和烯烃产生的决定因素。通过气相色谱/质谱法分析了[具体菌目]众多属的烯烃谱。我们首次描述了[具体属]、[具体属]、[具体属]、[具体属]、[具体属]、[具体属]、[具体属]、[具体属]和[具体属]代表菌株中的烯烃合成。通过将[具体菌]的天然[具体基因]与产生不同烯烃的放线菌的相应基因进行交换，我们证明了烯烃组成可以在链长和异构体组成方面进行调控。
本研究提供了一份在[具体菌门]中发现的烯烃结构多样性目录。我们的[具体基因]交换数据表明，烯烃结构从根本上由OleA的底物特异性决定，同时也由细胞脂肪酸代谢中足够数量的合适脂肪酰辅酶A底物的可用性决定。这使得革兰氏阳性菌的OleA成为结构分析和蛋白质工程的一个有前景的目标，旨在产生设计用于满足各种工业应用需求的烯烃链长和异构体谱。
