Razin S
J Bacteriol. 1968 Sep;96(3):687-94. doi: 10.1128/jb.96.3.687-694.1968.
The electrophoretic patterns of cell proteins in polyacrylamide gels were used for the study of several taxonomic problems in the Mycoplasmatales. The patterns of five Mycoplasma hominis strains showed marked differences that corresponded with their known serological and nucleic acid heterogeneity. The patterns of three M. mycoides var. mycoides strains isolated in different countries were essentially identical. The electrophoretic patterns of several caprine strains resembled those of M. mycoides var. mycoides, supporting their classification as M. mycoides var. capri. Strain B3, a swine isolate, accordingly was tentatively identified as M. mycoides var. capri. The bovine mastitis strain M. agalactiae var. bovis possessed a pattern basically similar to that of the goat mastitis strain M. agalactiae, supporting the inclusion of both strains in one species. Three M. pulmonis strains isolated from rats or tissue cultures showed nearly identical patterns. The pattern of the toxigenic M. neurolyticum (Sabin A) strain resembled but was not identical with that of the nontoxigenic PG28 strain. The avian Mycoplasma species, M. gallisepticum, M. meleagridis, M. synoviae, M. gallinarum, and M. iners showed easily distinguishable and specific patterns, supporting their present classification in different species. Several improvements in the electrophoretic technique are described, and its advantages and limitations as a taxonomic tool are discussed.
聚丙烯酰胺凝胶中细胞蛋白质的电泳图谱被用于研究支原体目中的几个分类学问题。五株人型支原体菌株的图谱显示出明显差异，这与它们已知的血清学和核酸异质性相对应。在不同国家分离出的三株丝状支原体丝状亚种菌株的图谱基本相同。几株山羊支原体菌株的电泳图谱与丝状支原体丝状亚种的图谱相似，支持将它们分类为丝状支原体山羊亚种。一株猪分离株B3因此被初步鉴定为丝状支原体山羊亚种。牛乳腺炎菌株无乳支原体牛亚种的图谱与山羊乳腺炎菌株无乳支原体的图谱基本相似，支持将这两种菌株归入同一个物种。从大鼠或组织培养物中分离出的三株肺支原体菌株显示出几乎相同的图谱。产毒素的溶神经支原体（萨宾A株）的图谱与无毒的PG28菌株的图谱相似但不完全相同。禽支原体物种，鸡毒支原体、火鸡支原体、滑膜支原体、鸡支原体和惰性支原体显示出易于区分的特异性图谱，支持它们目前在不同物种中的分类。文中描述了电泳技术的几项改进，并讨论了其作为分类工具的优缺点。
