用火箭免疫电泳法检测疫苗中的流感病毒结构抗原。
The assay of influenza virus structural antigens in vaccines by rocket immunoelectrophoresis.
作者信息
Oxford J S, Schild G C, Wood J M, Newman R W, Seagroatt V
出版信息
Dev Biol Stand. 1977;39:201-8.
PMID:604098
Abstract
The internally situated influenza virus structural antigens (matrix and nucleoprotein) and the glycoprotein hemagglutinin were assayed using rocket immunoelectrophoresis or single radial diffusion following disruption of purified virus with 1% w/v sodium sarcosyl detergent. Electrophoresis was carried out for 1-4 h in 0.05 M barbitone buffer at pH 8.6. The potency estimations of HA and M activity were similar for the two methods for bivalent beta-propiolactone inactivated whole virus vaccines.
摘要
使用1%（w/v）十二烷基肌氨酸钠去污剂裂解纯化病毒后，通过火箭免疫电泳或单向辐射扩散法检测内部存在的流感病毒结构抗原（基质蛋白和核蛋白）以及糖蛋白血凝素。电泳在pH 8.6的0.05 M巴比妥缓冲液中进行1 - 4小时。对于二价β-丙内酯灭活全病毒疫苗，两种方法对血凝素（HA）和基质蛋白（M）活性的效价估计相似。
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