人生长激素的氨基末端片段与人类绒毛膜促乳腺生长激素的羧基末端片段发生非共价相互作用，以产生促生长活性。
Noncovalent interaction of the NH2-terminal fragment of human somatotropin with the COOH-terminal fragment of human choriomammotropin to generate growth-promoting activity.
作者信息
Li C H
出版信息
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1700-2. doi: 10.1073/pnas.75.4.1700.
Abstract
Complementation of the plasmin fragments of reduced-carbamoylmethylated (Cam) human somatotropin (hGH) with those of reduced-carbamoylmethylated human chorionic somatomammotropin (hCS) have been investigated. It was found that the recombinant obtained by noncovalent interaction of [Cys(Cam)53]hGH-(1-134) with [Cys(Cam)-165,182,189]hCS-(141-191) exhibits 50% growth-promoting activity and nearly full immunoreactivity. Complementation of [Cys(Cam)53]hCS-(1-133) with the COOH-terminal fragment of hGH generated lower growth-promoting and immunological activities.
摘要
对还原氨甲酰甲基化（Cam）的人生长激素（hGH）的纤溶片段与还原氨甲酰甲基化的人绒毛膜生长催乳素（hCS）的纤溶片段的互补作用进行了研究。发现通过[Cys（Cam）53]hGH-（1-134）与[Cys（Cam）-165,182,189]hCS-（141-191）的非共价相互作用获得的重组体表现出50%的促生长活性和几乎完全的免疫反应性。[Cys（Cam）53]hCS-（1-133）与hGH的COOH末端片段的互补产生了较低的促生长活性和免疫活性。
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引用本文的文献
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Semisynthesis of human somatotropin analogs.人生长激素类似物的半合成
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6124-7. doi: 10.1073/pnas.76.12.6124.
2
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Proc Natl Acad Sci U S A. 1978 Nov;75(11):5391-4. doi: 10.1073/pnas.75.11.5391.
本文引用的文献
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Plasma growth hormone concentration in the rat determined by radioimmunoassay: influence of sex, pregnancy, lactation, anesthesia, hypophysectomy and extrasellar pituitary transplants.用放射免疫分析法测定大鼠血浆生长激素浓度：性别、妊娠、哺乳、麻醉、垂体切除及垂体鞍外移植的影响。
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Amino acid sequence of human chorionic somatomammotropin.人绒毛膜生长催乳素的氨基酸序列。
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