Rodkey L S
J Immunol. 1976 Sep;117(3):986-9.
Outbred rabbits were immunized with keyhole limpet hemocyanin diazotized to p-aminophenyl-N-trimethylammonium chloride (TMA). Anti-TMA antibodies were specifically purified and the F(ab')2 fragments were isolated after pepsin digestion. Glutaraldehyde-polymerized anti-TMA F(ab')2 fragments were reinjected back into the same animal that synthesized the antibodies to elicit an autologous anti-idiotypic (AAI) antiserum and were also injected into an allotypically matched recipient to elicit isologous anti-idiotypic (IAI) antiserum. AAI and IAI antisera reactive with the same F(ab')2 preparation were compared by assaying the populations of anti-TMA molecules reactive with each antiserum. The assays showed that the AAI antiserum reacted with smaller populations of molecules than did the IAI antiserum. The population of molecules recognized by the AAI antiserum was contained within the larger population recognized by the IAI antiserum. Hapten inhibition studies showed that the differences in the populations of molecules recognized by each antiserum were not related to the hapten-binding properties of the idiotypic antibodies: in one case both AAI and IAI reactions were equally inhibited by hapten; in another, the AAI reaction was inhibited more than was the IAI; in another, the IAI reaction was inhibited more.
用对氨基苯基 - N - 三甲基氯化铵（TMA）重氮化的钥孔戚血蓝蛋白免疫远交系兔。特异性纯化抗TMA抗体，并在胃蛋白酶消化后分离F(ab')2片段。将戊二醛聚合的抗TMA F(ab')2片段重新注射回合成抗体的同一只动物体内，以引发自体抗独特型（AAI）抗血清，同时也注射到同种异型匹配的受体中，以引发同种抗独特型（IAI）抗血清。通过检测与每种抗血清反应的抗TMA分子群体，比较与相同F(ab')2制剂反应的AAI和IAI抗血清。检测表明，AAI抗血清与比IAI抗血清更小的分子群体发生反应。AAI抗血清识别的分子群体包含在IAI抗血清识别的更大群体之中。半抗原抑制研究表明，每种抗血清识别的分子群体差异与独特型抗体的半抗原结合特性无关：在一种情况下，AAI和IAI反应均被半抗原同等程度地抑制；在另一种情况下，AAI反应比IAI反应受到的抑制更多；在另一种情况下，IAI反应受到的抑制更多。
