Thompson P J, Thames O A
Appl Microbiol. 1967 Sep;15(5):975-9. doi: 10.1128/am.15.5.975-979.1967.
A broth medium containing tryptone and manganese sulfate supported heavy sporulation of Bacillus stearothermophilus ATCC 7953 (NCA 1518) and four isolates identified as B. stearothermophilus. Maximal spore yields were obtained by use of inocula grown anaerobically in a medium containing glucose with aeration of sporulation medium via bubbling. After an extended stationary period, sporulation occurred concurrently with vegetative growth between 6 and 8 hr of incubation at 60 C. Omission of glucose from the inoculum or use of a "young" (2 hr) inoculum abolished the stationary period, but decreased spore yields. A requirement of oxygen for rapid vegetative growth and sporulation was demonstrated. Manganese (15 to 30 ppm) stimulated sporulation but did not enhance cell growth.
一种含有胰蛋白胨和硫酸锰的肉汤培养基支持嗜热脂肪芽孢杆菌ATCC 7953（NCA 1518）以及另外4株鉴定为嗜热脂肪芽孢杆菌的菌株大量产孢。通过使用在含葡萄糖的培养基中厌氧培养的接种物，并通过鼓泡对产孢培养基进行通气，可获得最大孢子产量。在延长的稳定期之后，在60℃培养6至8小时期间，产孢与营养生长同时发生。从接种物中省去葡萄糖或使用“年轻的”（2小时）接种物可消除稳定期，但会降低孢子产量。结果表明，快速的营养生长和产孢需要氧气。锰（15至30 ppm）刺激产孢，但不促进细胞生长。
