Murphy R A, Haque R
J Bacteriol. 1967 Nov;94(5):1327-33. doi: 10.1128/jb.94.5.1327-1333.1967.
Concentrated preparations of staphylococcal delta-hemolysin were obtained by growing selected hemolytic colonies from the 146P strain of Staphylococcus aureus on dialysis membranes laid over Brain Liver Heart agar plates at 37 C for 20 hr under 10% CO(2) and harvesting the growth from five such membranes in 1.0 ml of deionized distilled water. Incubation in a humid environment facilitated this harvesting procedure. Incubation longer than 40 hr or incubation under CO(2) higher than 10 to 20% gave lower yields of delta-lysin. Addition of a sugar, fermented by the organisms, resulted in lower yields of delta-hemolysin. Agar, although separated from the growing cells by the dialysis membrane, did potentiate delta-hemolysin production. Addition of 0.1% agar to the inoculum further enhanced this potentiation. delta-Hemolysin produced in broth or semisolid cultures was excessively diluted with the media. Dialysis membranes prevented this dilution and thus yielded concentrated preparations of delta-hemolysin.
通过在置于脑心肝琼脂平板上的透析膜上，于37℃、10%二氧化碳条件下培养金黄色葡萄球菌146P菌株的选定溶血菌落20小时，并从五块这样的膜上收获生长物，将其置于1.0毫升去离子蒸馏水中，获得了葡萄球菌δ-溶血素的浓缩制剂。在潮湿环境中孵育有助于此收获过程。孵育时间超过40小时或在高于10%至20%的二氧化碳条件下孵育，δ-溶血素的产量会降低。添加生物体可发酵的糖会导致δ-溶血素产量降低。琼脂虽然通过透析膜与生长的细胞分离，但确实会增强δ-溶血素的产生。向接种物中添加0.1%的琼脂可进一步增强这种增强作用。在肉汤或半固体培养物中产生的δ-溶血素会被培养基过度稀释。透析膜可防止这种稀释，从而产生δ-溶血素的浓缩制剂。
