Scherer R K, Brown R W
Appl Microbiol. 1974 Nov;28(5):768-73. doi: 10.1128/am.28.5.768-773.1974.
The electrophoretic separation of the proteinases produced by staphylococci and micrococci was studied in four buffers. The duration of electrophoresis was based on the migration of a marker dye for a predetermined distance. The migration distances of the enzymes and dye were measured, and enzyme-dye values were calculated. A comparison of enzyme-dye values showed that complete separation of eight serologically different proteinases did not occur in any one buffer; however, in most instances, their relative order of migration was the same in all buffers. Certain strains of Staphylococcus epidermidis produced two proteinases that were different serologically as well as electrophoretically. Staphylococcus aureus strains, on the other hand, produced up to four proteinases that were serologically the same. The proteinases of staphylococci and micrococci can be best characterized by both electrophoretic and serological methods.
在四种缓冲液中研究了葡萄球菌和微球菌产生的蛋白酶的电泳分离情况。电泳时间基于一种标记染料迁移预定距离来确定。测量了酶和染料的迁移距离，并计算了酶 - 染料值。酶 - 染料值的比较表明，在任何一种缓冲液中都无法实现八种血清学不同的蛋白酶的完全分离；然而，在大多数情况下，它们在所有缓冲液中的相对迁移顺序是相同的。某些表皮葡萄球菌菌株产生了两种在血清学和电泳方面都不同的蛋白酶。另一方面，金黄色葡萄球菌菌株产生了多达四种血清学相同的蛋白酶。葡萄球菌和微球菌的蛋白酶可以通过电泳和血清学方法进行最佳表征。
