粗球孢子菌小球体和关节孢子的血清学比较
Serological comparison of spherules and arthrospores of Coccidioides immitis.
作者信息
Landay M E, Wheat R W, Conant N F, Lowe E P
出版信息
J Bacteriol. 1967 Nov;94(5):1400-5. doi: 10.1128/jb.94.5.1400-1405.1967.
Abstract
Spherule and arthrospore cellular preparations were sonic-treated and separated into their respective supernatant and sediment components. Complement-fixation tests with antispherule and antiarthrospore pooled rabbit sera revealed that the soluble antigens exhibited more serological activity than the sediment preparations. After autoclaving, an arthrospore cellular antigen exhibited increased activity with either antisera, whereas autoclaved spherules exhibited increased activity only with antispherule serum. Complement-fixation tests with coccicioidin and spherule culture supernatant preparations revealed quantitative or qualitative differences in antigenic determinants between these two morphological phases of Coccidioides immitis.
摘要
对球形体和关节孢子细胞制剂进行超声处理，并分离成各自的上清液和沉淀成分。用抗球形体和抗关节孢子混合兔血清进行补体结合试验表明，可溶性抗原比沉淀制剂表现出更多的血清学活性。高压灭菌后，关节孢子细胞抗原与任何一种抗血清的活性均增加，而高压灭菌的球形体仅与抗球形体血清的活性增加。用球孢子菌素和球形体培养上清液制剂进行补体结合试验，揭示了粗球孢子菌这两个形态阶段之间抗原决定簇的定量或定性差异。
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本文引用的文献
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