轻度碱水解前后Vi抗原的理化性质
Physiochemical properties of the Vi antigen before and after mild alkaline hydrolysis.
作者信息
Jarvis F G, Mesenko M T, Martin D G, Perrine T D
出版信息
J Bacteriol. 1967 Nov;94(5):1406-10. doi: 10.1128/jb.94.5.1406-1410.1967.
Abstract
Sedimentation and diffusion constants were determined for electrophoretically purified Vi antigen before and after mild alkaline hydrolysis. The molecular weight of the intact antigen was found to be 1.7 x 10(6). Mild alkaline hydrolysis completely removed O-acetyl, reduced the molecular weight to 1.0 x 10(5), and lowered the intrinsic viscosity from 8.0 deciliters/g to approximately 0.5 deciliter/g. Infrared spectra of the two antigen preparations were also compared.
摘要
在温和碱性水解前后，对经电泳纯化的Vi抗原的沉降常数和扩散常数进行了测定。发现完整抗原的分子量为1.7×10⁶。温和碱性水解完全去除了O-乙酰基，使分子量降至1.0×10⁵，并使特性粘度从8.0分升/克降至约0.5分升/克。还比较了两种抗原制剂的红外光谱。
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