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轻度碱水解前后Vi抗原的理化性质

Physiochemical properties of the Vi antigen before and after mild alkaline hydrolysis.

作者信息

Jarvis F G, Mesenko M T, Martin D G, Perrine T D

出版信息

J Bacteriol. 1967 Nov;94(5):1406-10. doi: 10.1128/jb.94.5.1406-1410.1967.

DOI:10.1128/jb.94.5.1406-1410.1967
PMID:6057799
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC276839/
Abstract

Sedimentation and diffusion constants were determined for electrophoretically purified Vi antigen before and after mild alkaline hydrolysis. The molecular weight of the intact antigen was found to be 1.7 x 10(6). Mild alkaline hydrolysis completely removed O-acetyl, reduced the molecular weight to 1.0 x 10(5), and lowered the intrinsic viscosity from 8.0 deciliters/g to approximately 0.5 deciliter/g. Infrared spectra of the two antigen preparations were also compared.

摘要

在温和碱性水解前后，对经电泳纯化的Vi抗原的沉降常数和扩散常数进行了测定。发现完整抗原的分子量为1.7×10⁶。温和碱性水解完全去除了O-乙酰基，使分子量降至1.0×10⁵，并使特性粘度从8.0分升/克降至约0.5分升/克。还比较了两种抗原制剂的红外光谱。

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本文引用的文献

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8
An aminohexuronic acid as the principal hydrolytic component of the Vi antigen.一种氨基己糖醛酸作为Vi抗原的主要水解成分。
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Evidence for an aminohexuronic acid as hydrolytic product of Vi antigen.
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