脂质过氧化和2-烯丙基-2-异丙基乙酰胺导致细胞色素P-450中血红素的丧失。这是一条不涉及一氧化碳产生的异常途径。
Loss of haem from cytochrome P-450 caused by lipid peroxidation and 2-allyl-2-isoprophylacetamide. An abnormal pathway not involving production of carbon monoxide.
作者信息
De Matteis F, Gibbs A H, Unseld A
出版信息
Biochem J. 1977 Dec 15;168(3):417-22. doi: 10.1042/bj1680417.
Abstract
- Microsomal preparations undergoing lipid peroxidation produce CO and lose haem from cytochrome P-450. 2. The amount of CO produced does not correlate with the amount of haem lost and, after pre-labelling of microsomal haem in its bridges with 5-amino[5-14C]laevulinate, the radioactivity lost from haem is not recorved as CO. 3. Similarly, when pre-labelled microsomal haem is destroyed by the action of 2-allyl-2-isopropylacetamide, no radioactivity is recovered as CO. In clear contrast, on degradation of haem by the haem oxygenase system, CO is produced in an amount equimolar to the haem lost. 4. It is concluded that (a) the CO produced during lipid peroxidation originates from a source different from haem and (b) the degradations of haem caused by lipid peroxidation and 2-allyl-2-isopropylacetamide do not involve to any significant extent evolution of the methene-bridge carbon of haem as CO.
摘要
- 经历脂质过氧化的微粒体制剂会产生一氧化碳，并从细胞色素P-450中失去血红素。2. 产生的一氧化碳量与失去的血红素量不相关，并且在用5-氨基[5-¹⁴C]乙酰丙酸对微粒体血红素的桥进行预标记后，从血红素中损失的放射性不会以一氧化碳的形式回收。3. 同样，当预标记的微粒体血红素被2-烯丙基-2-异丙基乙酰胺作用破坏时，也没有放射性以一氧化碳的形式回收。相比之下，在血红素加氧酶系统降解血红素时，产生的一氧化碳量与失去的血红素量等摩尔。4. 得出的结论是：(a) 脂质过氧化过程中产生的一氧化碳源自与血红素不同的来源；(b) 脂质过氧化和2-烯丙基-2-异丙基乙酰胺引起的血红素降解在很大程度上不涉及血红素的亚甲基桥碳以一氧化碳的形式释放。
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