沙漠蝗（Schistocerca gregaria）中α-蜕皮激素C-20羟基化的机制。
The mechanism of C-20 hydroxylation of alpha-ecdysone in the desert locust, Schistocerca gregaria.
作者信息
Johnson P, Rees H H
出版信息
Biochem J. 1977 Dec 15;168(3):513-20. doi: 10.1042/bj1680513.
Abstract
- The C-20 hydroxylation of alpha-ecdysone to produce beta-ecdysone was investigated in the desert locust, Schistocerca gregaria. 2. alpha-Ecdysone C-20 hydroxylase activity was located primarily in the fat-body and Malpighian tubules. The properties of the hydroxylation system from Malpighian tubules investigated further. 3. The enzyme system was mitochondrial, had a pH optimum of 6.5, an apparent Km of 12.5 micron and required O2 and NADPH. 4. The activity of the hydroxylation system showed developmental variation within the fifth instar, the maximum activity corresponding to the maximum tire of endogenous moulting hormone. The significance of these results is assessed in relation to the control of the endogenous titre of beta-ecdysone. 5. The mechanism of the hydroxylation system was investigated by using known inhibitors of hydroxylation reactions such as CO, metyrapone and cyanide. 6. The CO difference spectrum of the reduced mitochondrial preparation indicated the presence of cytochrome P-450 in the preparation. 7. It concluded that the alpha-ecdysone C-20 hydroxylase system is a cytochrome P-450-deendent mono-oxygenase.
摘要
- 对沙漠蝗（Schistocerca gregaria）中α-蜕皮激素C-20位羟基化生成β-蜕皮激素的过程进行了研究。2. α-蜕皮激素C-20羟化酶活性主要位于脂肪体和马氏管中。对来自马氏管的羟基化系统的特性进行了进一步研究。3. 该酶系统存在于线粒体中，最适pH为6.5，表观Km为12.5微摩尔，需要氧气和NADPH。4. 羟基化系统的活性在五龄期内呈现发育变化，最大活性与内源性蜕皮激素的最高水平相对应。结合β-蜕皮激素内源性滴度的控制对这些结果的意义进行了评估。5. 通过使用已知的羟基化反应抑制剂如一氧化碳、甲吡酮和氰化物，对羟基化系统的机制进行了研究。6. 还原态线粒体制剂的一氧化碳差光谱表明制剂中存在细胞色素P-450。7. 得出结论：α-蜕皮激素C-20羟化酶系统是一种依赖细胞色素P-450的单加氧酶。
相似文献
1
The mechanism of C-20 hydroxylation of alpha-ecdysone in the desert locust, Schistocerca gregaria.
Biochem J. 1977 Dec 15;168(3):513-20. doi: 10.1042/bj1680513.
2
Ecdysone 20-mono-oxygenase in the desert locust, Schistocerca gregaria.
Biochem J. 1984 Nov 1;223(3):837-47. doi: 10.1042/bj2230837.
3
Ecdysterone biosynthesis: a microsomal cytochrome-P-450-linked ecdysone 20-monooxygenase from tissues of the African migratory locust.
Eur J Biochem. 1978 Jul 17;88(1):37-47. doi: 10.1111/j.1432-1033.1978.tb12420.x.
4
RNAi-mediated knockdown of Shade negatively affects ecdysone-20-hydroxylation in the desert locust, Schistocerca gregaria.
J Insect Physiol. 2012 Jul;58(7):890-6. doi: 10.1016/j.jinsphys.2012.03.013. Epub 2012 Mar 29.
5
Mechanism of hydroxylation at C-2 during the biosynthesis of ecdysone in ovaries of the locust, Schistocerca gregaria.
Biochem J. 1984 Feb 1;217(3):783-9. doi: 10.1042/bj2170783.
6
Development of microsomal cytochrome P-450 monooxygenases during the last larval instar of the locust, Locusta migratoria: correlation with the hemolymph 20-hydroxyecdysone titer.
Mol Cell Endocrinol. 1980 Nov;20(2):157-69. doi: 10.1016/0303-7207(80)90079-9.
7
A microsomal ecdysone-binding cytochrome P450 from the insect Locusta migratoria purified by sequential use of type-II and type-I ligands.
Biol Chem. 2001 Nov;382(11):1541-9. doi: 10.1515/BC.2001.188.
8
Dynamics of ecdysone metabolism after ingestion and injection in Locusta migratoria.
Gen Comp Endocrinol. 1976 Jul;29(3):319-27. doi: 10.1016/0016-6480(76)90044-7.
9
Conversion of a radiolabelled ecdysone precursor, 2,22,25-trideoxyecdysone, by embryonic and larval tissues of Locusta migratoria.
Mol Cell Endocrinol. 1985 Jun;41(1):27-44. doi: 10.1016/0303-7207(85)90140-6.
10
Formation of the A/B cis ring junction of ecdysteroids in the locust, Schistocerca gregaria.
Biochem J. 1981 Jan 15;194(1):53-62. doi: 10.1042/bj1940053.
引用本文的文献
1
Identification of genes and gene expression associated with dispersal capacity in the mountain pine beetle, Hopkins (Coleoptera: Curculionidae).
PeerJ. 2021 Oct 26;9:e12382. doi: 10.7717/peerj.12382. eCollection 2021.
2
Role of ecdysone 20-monooxygenase in regulation of 20-hydroxyecdysone levels by juvenile hormone and biogenic amines in Drosophila.
J Comp Physiol B. 2008 Jan;178(1):27-32. doi: 10.1007/s00360-007-0196-x. Epub 2007 Aug 17.
3
Immunological analysis of developmental changes in ecdysone 20-mono-oxygenase expression in the cotton leafworm, Spodoptera littoralis.
Biochem J. 1994 May 1;299 ( Pt 3)(Pt 3):711-7. doi: 10.1042/bj2990711.
4
Ecdysone 20-mono-oxygenase in the desert locust, Schistocerca gregaria.
Biochem J. 1984 Nov 1;223(3):837-47. doi: 10.1042/bj2230837.
5
Effects of the chitin synthetase inhibitor plumbagin and its 2-demethyl derivative juglone on insect ecdysone 20-monooxygenase activity.
Experientia. 1988 Dec 1;44(11-12):990-1. doi: 10.1007/BF01939896.
6
Effects of the adenylate cyclase activator forskolin and its inactive derivative 1,9-dideoxyforskolin on insect cytochrome P-450 dependent steroid hydroxylase activity.
Experientia. 1992 Jan 15;48(1):39-41. doi: 10.1007/BF01923603.
本文引用的文献
1
Biosynthesis of agr-Ecdysone by Prothoracic Glands in vitro.
Science. 1974 Feb 8;183(4124):529-30. doi: 10.1126/science.183.4124.529.
2
The Secretion of alpha-Ecdysone by the Prothoracic Glands of Manduca sexta In Vitro.
Proc Natl Acad Sci U S A. 1974 Mar;71(3):793-6. doi: 10.1073/pnas.71.3.793.
3
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
4
THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. II. SOLUBILIZATION, PURIFICATION, AND PROPERTIES.
J Biol Chem. 1964 Jul;239:2379-85.
5
Pharmacology and biochemistry of some amphenone analogues and other adrenal cortical inhibitors.
J Med Pharm Chem. 1959 Oct;1:407-41. doi: 10.1021/jm50006a002.
6
The cholesterol side-chain cleavage system of bovine adrenal cortex.
Eur J Biochem. 1967 Oct;2(3):275-85. doi: 10.1111/j.1432-1033.1967.tb00136.x.
7
Aldosterone synthesis by adrenal mitochondria. 3. Participation of cytochrome P-450.
Arch Biochem Biophys. 1967 Aug;121(2):298-303. doi: 10.1016/0003-9861(67)90079-3.
8
Studies on succinate dehydrogenase. IV. Kinetics of the overall reaction catalysed by preparations of the purified enzyme.
Biochim Biophys Acta. 1969 Apr 22;178(2):213-24. doi: 10.1016/0005-2744(69)90391-x.
9
On the origin and metabolic fate of alpha-ecdysone in insects.
Gen Comp Endocrinol. 1970 Aug;15(1):80-7. doi: 10.1016/0016-6480(70)90099-7.
10
Studies on adrenal cortical cytochrome P-450. 3. Effects of carbon monoxide and light on steroid 11-beta hydroxylation.
Biochemistry. 1970 Mar 31;9(7):1615-21. doi: 10.1021/bi00809a021.
Zotero 插件