飞蝗（沙漠蝗）中蜕皮甾体A/B顺式环连接的形成
Formation of the A/B cis ring junction of ecdysteroids in the locust, Schistocerca gregaria.
作者信息
Davies T G, Dinan L N, Lockley W J, Rees H H, Goodwin T W
出版信息
Biochem J. 1981 Jan 15;194(1):53-62. doi: 10.1042/bj1940053.
Abstract
- The mechanism of formation of the A/B cis ring junction of ecdysteroids in the locust Schistocerca gregaria, was investigated by incorporation of [4-14C,3 alpha-3H], [4-14C,4 alpha-3H] and [4-14C,4 beta-3H]cholesterol into 20-hydroxyecdysone in fifth-instar larvae and into ecdysteroid conjugates in ovaries of maturing adult females. 2. In both systems there was retention of the 4 alpha-3H atom in the ecdysteroid and elimination of the 3 alpha- and 4 beta-3H atoms. 3. The 3H retained in the ecdysone formed from [4 alpha-3H]cholesterol in the ovarian system was probably located at C-4. The results are interpreted by postulating the involvement of a 3-oxo-delta 4 intermediate in ecdysteroid biosynthesis in insects.
摘要
- 通过将[4-¹⁴C,3α-³H]、[4-¹⁴C,4α-³H]和[4-¹⁴C,4β-³H]胆固醇掺入五龄幼虫的20-羟基蜕皮激素以及成熟成年雌性蝗虫卵巢中的蜕皮甾类结合物中，研究了蝗虫（沙漠蝗）中蜕皮甾类A/B顺式环连接的形成机制。2. 在这两个系统中，蜕皮甾类中4α-³H原子得以保留，而3α-和4β-³H原子则被消除。3. 在卵巢系统中由[4α-³H]胆固醇形成的蜕皮激素中保留的³H可能位于C-4位。通过假设3-氧代-δ⁴中间体参与昆虫蜕皮甾类生物合成来解释这些结果。
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