Pirtle E C
J Infect Dis. 1977 Dec;136 Suppl:S703-5. doi: 10.1093/infdis/136.supplement_3.s703.
Twenty-one pigs delivered by hysterectomy from three sows were used to evaluate influenza A/New Jersey/76 virus vaccine in neonatal pigs. Pigs were vaccinated on the day of birth or at three days of age with either 200 or 400 chick cell-agglutinating (CCA) units of vaccine, and pigs were bled two and four weeks after vaccination. The 21 vaccinated pigs and two unvaccinated control pigs were challenged intranasally at five weeks of age, and nasal swabs were obtained for six consecutive days after challenge. Two weeks after vaccination, only three pigs had reciprocal hemagglutination-inhibiting (HAI) antibody titers of greater than or equal to 20. Four weeks after vaccination, 11 pigs had HAI titers of greater than or equal to 20, and titers of virus-neutralizing antibody ranged from less than 10 to 1,000 among these same sera. On day 7 after challenge, 12 pigs had HAI titers of greater than or equal to 20. Swine-like influenza virus was isolated from nasal swabs from the controls and from four of the 21 vaccinated pigs. The results indicated that 80% of the vaccinated pigs were protected.
从三头母猪经子宫切除术分娩的21头仔猪用于评估甲型流感/新泽西/76病毒疫苗对新生仔猪的效果。仔猪在出生当天或三日龄时接种200或400鸡胚细胞凝集（CCA）单位的疫苗，并在接种后两周和四周采血。21头接种疫苗的仔猪和2头未接种疫苗的对照仔猪在五周龄时进行鼻内攻毒，并在攻毒后连续六天采集鼻拭子。接种疫苗两周后，只有三头仔猪的血凝抑制（HAI）抗体效价大于或等于20。接种疫苗四周后，11头仔猪的HAI效价大于或等于20，并且在这些相同血清中病毒中和抗体效价范围为小于10至1000。攻毒后第7天，12头仔猪的HAI效价大于或等于20。从对照猪和21头接种疫苗仔猪中的4头的鼻拭子中分离出猪流感病毒。结果表明80%的接种疫苗仔猪受到保护。
