Bikour M H, Cornaglia E, Elazhary Y
Virology Section, Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Québec.
Can J Vet Res. 1996 Oct;60(4):312-4.
A challenge study was conducted to evaluate the safety and efficacy of an inactivated influenza H3N2 virus vaccine combined with Quil A/Alhydrogel mixture under controlled conditions in piglets. Twenty-four piglets from 12 sows were allocated to 2 groups; injected intramuscularly with 2 doses of the tested vaccine or with PBS at 2 wk intervals and challenged intratracheally with 105TCID50 of the H3N2 swine influenza virus 6 d after the 2nd immunization. Clinical and virological parameters were recorded for 4 d after the challenge. The use of the tested vaccine produced high serum hemagglutination-inhibition titers against the swine H3N2 strain virus. This strong immune response suppressed all clinical signs and viral shedding and reduced pulmonary lesions due to the challenge in the vaccinated group, without causing any secondary effects. Our results suggest that the serum HI titers correlated with the degree of protection induced by an inactivated swine influenza H3N2 vaccine.
进行了一项攻毒试验研究，以评估在受控条件下仔猪中一种灭活H3N2流感病毒疫苗与Quil A/氢氧化铝凝胶混合物联合使用的安全性和有效性。将来自12头母猪的24只仔猪分为2组；每隔2周肌肉注射2剂受试疫苗或PBS，并在第二次免疫后6天经气管内接种105TCID50的H3N2猪流感病毒。攻毒后4天记录临床和病毒学参数。使用受试疫苗产生了针对猪H3N2毒株病毒的高血清血凝抑制效价。这种强烈的免疫反应抑制了所有临床症状和病毒排出，并减少了接种组因攻毒引起的肺部病变，且未产生任何副作用。我们的结果表明，血清HI效价与灭活猪H3N2流感疫苗诱导的保护程度相关。
