一种用于测定痕量标记抗体相对亲和力的分析方法。
An assay for determining the relative affinity of trace amounts of labeled antibodies.
作者信息
Steiner L A, Eisen H N
出版信息
J Exp Med. 1967 Dec 1;126(6):1143-60. doi: 10.1084/jem.126.6.1143.
Abstract
All assay for the binding efficiency or "relative affinity" of trace amounts of radioactively labeled anti-2,4-dinitrophenyl (DNP) antibodies has been developed. The assay measures the relative ability of the labeled antibodies to combine and precipitate with antigen in the presence of a large amount of unlabeled reference antibody of the same specificity. It has been possible to correlate the relative affinity of the labeled antibodies for dinitrophenylated antigens with the association constants of the reference antibodies for simple univalent DNP ligands.
摘要
已开发出所有用于检测微量放射性标记抗2,4 -二硝基苯基（DNP）抗体的结合效率或“相对亲和力”的检测方法。该检测方法测量在存在大量相同特异性的未标记参考抗体的情况下，标记抗体与抗原结合并沉淀的相对能力。已能够将标记抗体对二硝基苯基化抗原的相对亲和力与参考抗体对简单单价DNP配体的缔合常数相关联。
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引用本文的文献
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The relative affinity of antibodies synthesized in the secondary response.二次免疫应答中合成的抗体的相对亲和力。
J Exp Med. 1967 Dec 1;126(6):1185-205. doi: 10.1084/jem.126.6.1185.
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