Bornside G H
Appl Microbiol. 1967 Sep;15(5):1020-4. doi: 10.1128/am.15.5.1020-1024.1967.
Growth of Staphylococcus aureus ATCC 6538P was studied in stationary broth cultures (11 mm deep) exposed to hyperbaric oxygen (100% O(2) at 3 atm absolute). The minimal inhibitory concentration (MIC) of the following antibiotics was determined after exposure to high-pressure oxygen (HPO) for 3, 6, and 12 hr: penicillin, streptomycin, tetracycline, oxytetracycline, kanamycin, and cephalothin. Logarithmic growth during exposure to HPO was retarded 60%. Air at 3 atm absolute did not retard growth. The longer the exposure of tube dilution tests to HPO, the lower the MIC. Regardless of the antibiotic used, MIC values relative to 100% for unexposed controls were similar for given exposures, and averaged 73% after 3 hr of exposure to HPO, 53% after 6 hr, and 34% after 12 hr. Similar enhancement with HPO and an iodophor suggests occurrence of a general phenomenon with antibacterial agents. Although HPO alone is primarily bacteriostatic, combined therapy with antibiotics and HPO may be useful against bacterial infections because the therapeutic effectiveness of a maximal dosage of antibiotic could be increased.
在深度为11毫米的静止肉汤培养物中，研究了金黄色葡萄球菌ATCC 6538P在高压氧（绝对压力3个大气压下的100% O₂）环境中的生长情况。在暴露于高压氧（HPO）3小时、6小时和12小时后，测定了以下抗生素的最低抑菌浓度（MIC）：青霉素、链霉素、四环素、土霉素、卡那霉素和头孢菌素。暴露于HPO期间的对数生长期生长延缓了60%。绝对压力3个大气压的空气不会延缓生长。试管稀释试验暴露于HPO的时间越长，MIC越低。无论使用何种抗生素，对于给定的暴露时间，相对于未暴露对照的100%而言，MIC值相似，暴露于HPO 3小时后平均为73%，6小时后为53%，12小时后为34%。HPO和碘伏有类似的增强作用，提示抗菌剂存在一种普遍现象。虽然单独的HPO主要是抑菌性的，但抗生素与HPO联合治疗可能对细菌感染有用，因为最大剂量抗生素的治疗效果可能会提高。
