Kejzlarová-Lepesant J, Brock H W, Moreau J, Dubertret M L, Billault A, Lepesant J A
Nucleic Acids Res. 1984 Dec 11;12(23):8835-46. doi: 10.1093/nar/12.23.8835.
A phage containing two sequences homologous to U1 snRNA was isolated from a Drosophila melanogaster genomic library, and identified with a previously cloned D. melanogaster U1 snRNA gene. DNA sequence analysis showed that complete and truncated U1 snRNA genes are present, both of which have base substitutions relative to U1 snRNA. These genes show conservation of 5' and 3' flanking regions relative to other U1 and U2 snRNA genes of Drosophila. Intramolecular renaturation experiments and electron microscope mapping demonstrates that the two U1 snRNA sequences are present as inverted repeats about 2.7kb apart, separated by a smaller pair of inverted repeats of an unrelated sequence. These U1 snRNA sequences were located by in situ hybridization at 82E, and related sequences were found at 21D and 95C on the polytene chromosome map. The results are discussed with reference to the origin and function of snRNAs.
从黑腹果蝇基因组文库中分离出一个含有两个与U1 snRNA同源序列的噬菌体，并用先前克隆的黑腹果蝇U1 snRNA基因进行鉴定。DNA序列分析表明存在完整和截短的U1 snRNA基因，两者相对于U1 snRNA都有碱基替换。相对于果蝇的其他U1和U2 snRNA基因，这些基因在5'和3'侧翼区域表现出保守性。分子内复性实验和电子显微镜图谱显示，两个U1 snRNA序列以反向重复的形式存在，相距约2.7kb，由一对较小的无关序列的反向重复隔开。这些U1 snRNA序列通过原位杂交定位在82E，在多线染色体图谱上的21D和95C处发现了相关序列。并参考snRNAs的起源和功能对结果进行了讨论。
