Myslinski E, Branlant C, Wieben E D, Pederson T
J Mol Biol. 1984 Dec 25;180(4):927-45. doi: 10.1016/0022-2836(84)90264-x.
We have investigated the sequences of the major small nuclear RNAs of Drosophila cultured cells, with the objective of elucidating phylogenetically conserved primary and secondary structures by comparison of the data with previously determined sequences of these RNAs in vertebrate species. Our results reveal striking degrees of conservation between each Drosophila RNA and its vertebrate cognate, and also demonstrate blocks of homology among the Drosophila small nuclear RNAs, as previously described for vertebrates. The most conserved features include the 5' terminal region of U1 RNA, though to function in pre-mRNA splicing, most of the regions of U4 RNA recently implicated in 3' processing of pre-mRNA, and the major snRNP protein binding site ("domain A") that is also shared by vertebrate U1, U2, U4 and U5 RNAs. Several other conserved features have been revealed, suggesting additional regions of functional significance in these RNAs and also providing further insights into the evolutionary history of the small nuclear RNAs.
我们研究了果蝇培养细胞中主要小核RNA的序列，目的是通过将这些数据与脊椎动物物种中先前确定的这些RNA序列进行比较，阐明系统发育上保守的一级和二级结构。我们的结果揭示了每种果蝇RNA与其脊椎动物同源物之间惊人的保守程度，并且还证明了果蝇小核RNA之间的同源区域，正如先前对脊椎动物所描述的那样。最保守的特征包括U1 RNA的5'末端区域（尽管其在mRNA前体剪接中起作用）、最近涉及mRNA前体3'加工的U4 RNA的大部分区域，以及脊椎动物U1、U2、U4和U5 RNA也共有的主要snRNP蛋白结合位点（“结构域A”）。还发现了其他几个保守特征，这表明这些RNA中还有其他具有功能意义的区域，也为小核RNA的进化历史提供了进一步的见解。
