Oehling A, Ona J, Trento H, Sanz M L, Domínguez M A
Allergol Immunopathol (Madr). 1984 Nov-Dec;12(6):439-48.
One hundred and nine patients were selected in this study. All had food allergies (Urticaria, Quincke's Oedema and Bronchial Asthma) diagnosed by either clinical history or skin test results later confirmed by oral challenge. Since many of these patients had multiple sensitivities to various food allergens, the study was performed on 175 blood samples classifying the results according to personal history and results of the skin test. The diagnostic value of histamine release in whole blood of a patient suffering from food allergy is optimally reliable. The study is complemented by providing a better understanding of the dynamics involved in the hypersensitivity response. The results from this comparative study with RAST, once again demonstrate the uselessness in using this technique in food allergy since the existence of other cytotropic antibodies, IgG4 capable of provoking the basophil to produce a clearly detectable and significant histamine release has been well demonstrated. Once again the importance of other antibodies which are not exactly cytotropic or anaphylactic (IgG, IgM, etc.) in nature in hypersensitivity response is brought to light. These are readily detected with a great degree of reliability (78%) using hemagglutination. This method is highly sensitive and is strongly recommended in the diagnoses of allergy because of its simplicity and affordability. The study of the parameters studied shows us once again that there are several mechanisms involved in the hypersensitivity response as well as a variation from one individual to another depending, of course on the class of antigen. Finally we must keep in mind the complexity of the antigen involved in food allergies which in many cases are haptenic.
本研究选取了109名患者。所有患者均患有食物过敏（荨麻疹、昆克水肿和支气管哮喘），通过临床病史或皮肤试验结果诊断，随后经口服激发试验确诊。由于这些患者中有许多对多种食物过敏原具有多种敏感性，因此对175份血样进行了研究，并根据个人病史和皮肤试验结果对结果进行分类。食物过敏患者全血中组胺释放的诊断价值具有最佳可靠性。该研究通过更好地理解超敏反应所涉及的动态过程得到了补充。这项与放射变应原吸附试验（RAST）的对比研究结果再次表明，在食物过敏中使用该技术毫无用处，因为已经充分证明存在其他嗜细胞抗体，即IgG4，它能够刺激嗜碱性粒细胞产生明显可检测到的显著组胺释放。超敏反应中本质上并非完全嗜细胞性或过敏性的其他抗体（IgG、IgM等）的重要性再次被揭示出来。使用血凝反应能够高度可靠地（78%）检测到这些抗体。该方法高度灵敏，因其简单且经济实惠，在过敏诊断中强烈推荐使用。对所研究参数的研究再次向我们表明，超敏反应涉及多种机制，并且个体之间存在差异，当然这取决于抗原类别。最后，我们必须牢记食物过敏中所涉及抗原的复杂性，在许多情况下这些抗原是半抗原。
