Southgate K, Creagh F, Teece M, Kingswood C, Rees Smith B
Clin Endocrinol (Oxf). 1984 May;20(5):539-48. doi: 10.1111/j.1365-2265.1984.tb00102.x.
A receptor assay for TSH receptor antibodies is described in which unextracted serum, detergent solubilised TSH receptors and 125I-labelled TSH are used. The assay was rapid and reproducible with coefficients of inter-assay variation of 12.3%, 2.1 and 2.6% at mean inhibition of TSH binding values of 11, 53, and 79 respectively. Assay sensitivity could be increased by reducing the volume of receptors used but some increase in the scatter of values obtained with individual normal sera was also observed. Comparison of human and porcine TSH receptor preparations indicated that porcine tissue gave greater sensitivity. Analysis of different groups of patients and normal subjects (n = 21) showed the absence of detectable TSH receptor antibody activity in 16 patients with rheumatoid arthritis, 10 with multinodular goitre and 12 with Hashimoto's disease. However the antibody was readily detectable in 28 out of 28 Graves' patients (treated and untreated) who were hyperthyroid at the time of assay.
本文描述了一种促甲状腺激素（TSH）受体抗体的检测方法，该方法使用未提取的血清、经去污剂溶解的TSH受体和125I标记的TSH。该检测方法快速且可重复，在TSH结合值平均抑制率分别为11%、53%和79%时，批间变异系数分别为12.3%、2.1%和2.6%。通过减少所用受体的体积可提高检测灵敏度，但同时也观察到个别正常血清所得值的离散度有所增加。对人源和猪源TSH受体制剂的比较表明，猪组织具有更高的灵敏度。对不同组患者和正常受试者（n = 21）的分析显示，16例类风湿性关节炎患者、10例结节性甲状腺肿患者和12例桥本氏病患者中均未检测到可检测的TSH受体抗体活性。然而，在检测时甲状腺功能亢进的28例格雷夫斯病患者（包括接受治疗和未接受治疗的）中，有28例均能轻易检测到该抗体。
