Andrieux B
Gen Comp Endocrinol. 1984 Jul;55(1):74-82. doi: 10.1016/0016-6480(84)90130-8.
In the female newt, Pleurodeles waltlii, the somatotropic cells of the adenohypophysis are characterized by large, electron-dense inclusions (1-6 micron). Inclusions were also found in adult male newts after treatment with 17-beta-estradiol (5 mg/liter of aquarium water, for 10 days to 4 months). The ultrastructural genesis of these inclusions is described for treated males and for females during sexual maturation. In both cases, the same morphological features (dimensions, and filamentous and crystalloid arrangements) have been observed. The origin of the inclusions is discussed with respect to estrogens. The effects of 17-beta-estradiol are also studied in gonadotropic pituitary cells (GTH cells) of the male frog (Rana temporaria) after injections ranging over 6 to 8 weeks (0.05 or 0.5 mg/0.1 cc PPG) every 4 days. In GTH cells, aggregates of tubules are present in large inclusions. Such aggregates are observed in GTH cells in the female frog 8 to 10 weeks after breeding. The aggregates are thought to be involved in the process of intracellular regulation of hormone secretion, since such structures appear when there is a high level of circulating estrogens.
在雌性疣螈（Pleurodeles waltlii）中，腺垂体的促生长细胞具有特征性的大的、电子致密包涵体（1 - 6微米）。在用17-β-雌二醇（5毫克/升水族箱水，处理10天至4个月）处理后的成年雄性疣螈中也发现了包涵体。本文描述了在性成熟过程中，处理后的雄性和雌性疣螈中这些包涵体的超微结构起源。在这两种情况下，都观察到了相同的形态特征（尺寸、丝状和晶体排列）。文中讨论了雌激素与包涵体起源的关系。还对雄性青蛙（林蛙，Rana temporaria）每隔4天注射6至8周（0.05或0.5毫克/0.1毫升聚丙二醇）后，促性腺垂体细胞（GTH细胞）中17-β-雌二醇的作用进行了研究。在GTH细胞中，大的包涵体内存在小管聚集体。在雌性青蛙繁殖后8至10周的GTH细胞中也观察到了这种聚集体。这些聚集体被认为参与了激素分泌的细胞内调节过程，因为当循环雌激素水平较高时会出现这样的结构。
