Buck G A, Cross R E, Wong T P, Loera J, Groman N
Infect Immun. 1985 Sep;49(3):679-84. doi: 10.1128/iai.49.3.679-684.1985.
The DNA genomes of a number of tox-bearing, temperate corynebacteriophages isolated from strains of Corynebacterium diphtheriae and Corynebacterium ulcerans were compared. With one exception, these phages displayed similarities in their restriction enzyme digest profiles and extensive homology with prototypic beta converting phage. The exception, phage delta, had a unique restriction profile and exhibited homology with beta over a limited portion of its genome. DNAs of phages from each host contained cohesive ends and integrated as prophage by a mechanism analogous to that employed by coliphage lambda. It is proposed that these tox-bearing phages belong to a common family, the beta family. The role of the beta family in the movement of the tox gene between strains of C. diphtheriae and C. ulcerans is discussed.
对从白喉棒状杆菌和溃疡棒状杆菌菌株中分离出的多种携带毒素的温和棒状噬菌体的DNA基因组进行了比较。除了一个例外，这些噬菌体在其限制性酶切图谱上表现出相似性，并且与原型β转化噬菌体具有广泛的同源性。例外的是δ噬菌体，它具有独特的限制性图谱，并且在其基因组的有限部分与β噬菌体表现出同源性。来自每个宿主的噬菌体DNA都含有粘性末端，并通过类似于大肠杆菌噬菌体λ所采用的机制作为原噬菌体整合。有人提出，这些携带毒素的噬菌体属于一个共同的家族，即β家族。讨论了β家族在白喉棒状杆菌和溃疡棒状杆菌菌株之间毒素基因转移中的作用。
