The genomes of rotaviruses isolated from chronically infected immunodeficient children.

Abnormal RNA migration profiles were found in numerous rotavirus samples from two chronically infected children suffering from severe combined immunodeficiency. In both cases additional bands consisting of dsRNA were found migrating between RNA segments 1 and 7, and in one case RNA segment 11 was lost from the profile. Hybridization studies using segment-specific cloned cDNA probes indicated that some of the additional bands had sequence homologies with normal rotavirus dsRNA segments. In most cases these sequences were derived from genome segments of lower molecular weight by the formation of covalently linked concatemers.