Yamaguchi T, Hayashi K, Murakami H, Maruyama S, Yamaguchi M
Neurochem Res. 1984 Apr;9(4):497-505. doi: 10.1007/BF00964376.
In a search for neurochemical involvement in cerebellar ataxia, Rolling Mouse Nagoya (C3Hf/Nem-rol), which shows only hypoplasia of the cerebellum but no pathological configuration of the cerebellar structure, was used to study glutamate receptors in the CNS. Kainic acid binding sites were significantly decreased in the thalamus, hypothalamus, pons, and cerebellum, and in the frontal cortex of both the ataxic mutant mouse and the non-ataxic heterozygote. Only spinal cord and midbrain of the ataxic mutant mouse showed decreased distribution of kainate binding sites in the membrane fraction. Among enzymes responsible for supplying glutamate to the receptor, GDH showed higher activity in the spinal cord of the ataxic mutant mouse.
为了探究神经化学物质与小脑共济失调的关系，选用了滚动小鼠名古屋品系（C3Hf/Nem-rol），该品系仅表现出小脑发育不全，但小脑结构无病理改变，用于研究中枢神经系统中的谷氨酸受体。在丘脑、下丘脑、脑桥、小脑以及共济失调突变小鼠和非共济失调杂合子的额叶皮质中，海人酸结合位点显著减少。仅共济失调突变小鼠的脊髓和中脑在膜部分显示出海人酸盐结合位点的分布减少。在负责为受体提供谷氨酸的酶中，谷氨酸脱氢酶（GDH）在共济失调突变小鼠的脊髓中活性较高。
