Valentin-Hansen P, Hammer K, Løve Larsen J E, Svendsen I
Nucleic Acids Res. 1984 Jul 11;12(13):5211-24. doi: 10.1093/nar/12.13.5211.
Previous studies of the structure and regulation of the deo operon in Escherichia coli have localized an internal regulated promoter, called deoP3, in front of the two distal genes in the operon. We report here the nucleotide sequence of the distal portion of the deoA, the deoA-deoB intercistronic region and the first part of the deoB gene, and show that deoP3 overlaps the distal segment of the deoA gene. The location of the internal promoter and the transcriptional start site were determined by means of 1) sequence homology to the consensus promoter sequence of E. coli, 2) high resolution S1 nuclease mapping of in vivo transcripts and 3) in vivo regulation of beta-galactosidase from low as well as high copy number P31acZ protein fusion vectors.
先前对大肠杆菌中deo操纵子的结构和调控的研究已将一个内部调控启动子（称为deoP3）定位在该操纵子中两个远端基因的前方。我们在此报告deoA远端部分、deoA-deoB基因间区域以及deoB基因第一部分的核苷酸序列，并表明deoP3与deoA基因的远端片段重叠。内部启动子的位置和转录起始位点是通过以下方法确定的：1）与大肠杆菌共有启动子序列的序列同源性；2）体内转录本的高分辨率S1核酸酶图谱分析；3）来自低拷贝数和高拷贝数P31acZ蛋白融合载体的β-半乳糖苷酶的体内调控。
