来自嗜热栖热甲烷球菌的三种新型限制性内切酶MaeI、MaeII和MaeIII。
Three new restriction endonucleases MaeI, MaeII and MaeIII from Methanococcus aeolicus.
作者信息
Schmid K, Thomm M, Laminet A, Laue F G, Kessler C, Stetter K O, Schmitt R
出版信息
Nucleic Acids Res. 1984 Mar 26;12(6):2619-28. doi: 10.1093/nar/12.6.2619.
Three type II restriction endonucleases, MaeI, MaeII and MaeIII, with novel site specificities have been isolated and purified from the archaebacterium Methanococcus aeolicus PL-15/H. The recognition sequences of these enzymes are (formula: see text) with the sites of cleavage as indicated by the arrows. The sequences were confirmed by restriction and computer analyses on sequenced DNA's of plasmid pBR322, bacteriophages lambda and phi X174 and virus SV40.
已从嗜热栖甲烷球菌PL-15/H中分离并纯化出三种具有新型位点特异性的II型限制性核酸内切酶MaeI、MaeII和MaeIII。这些酶的识别序列为（公式：见原文），其切割位点如箭头所示。通过对质粒pBR322、噬菌体λ和φX174以及病毒SV40的测序DNA进行限制性酶切和计算机分析，证实了这些序列。
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