Yasuda S, Furuichi M, Soeda E
Nucleic Acids Res. 1984 Jul 25;12(14):5583-8. doi: 10.1093/nar/12.14.5583.
The DNA fragment encompassing the ras gene of Harvey murine sarcoma virus was sequenced and assigned the coding region of a transforming protein, p21, to the sequence. Examination of nucleotide sequence, taken together with the result of analysis of the ras mRNAs (1), has revealed that p21 is encoded from a continuous coding region starting with the 5' proximal initiation codon but not a processed protein. However, there were found several differences between the sequence published by Dhar et. al. (2) and ours, including 9 deletions, 7 substitutions and 2 insertions of nucleotides in the published sequence of 997 nucleotides in length. Among these, one of the substitutions occurring in the coding region resulted in amino acid replacement of glycine by alanine at position 122 of p21. The evidences are presented with some of actual gel autoradiographs.
对包含哈维鼠肉瘤病毒ras基因的DNA片段进行了测序，并将一种转化蛋白p21的编码区定位到该序列上。对核苷酸序列的检查，结合ras mRNA的分析结果(1)，表明p21是由一个从5'近端起始密码子开始的连续编码区编码的，而不是一种加工后的蛋白。然而，在Dhar等人(2)发表的序列与我们的序列之间发现了几个差异，包括在长度为997个核苷酸的已发表序列中有9个缺失、7个替换和2个核苷酸插入。其中，编码区发生的一个替换导致p21第122位的甘氨酸被丙氨酸取代。文中展示了一些实际的凝胶放射自显影片作为证据。
