Hearing J C, Nicolas J C, Levine A J
Proc Natl Acad Sci U S A. 1984 Jul;81(14):4373-7. doi: 10.1073/pnas.81.14.4373.
The Epstein-Barr virus (EBV) BamHI restriction endonuclease fragment K (B95-8 strain) was introduced into a polyoma virus expression vector and used to transfect murine NIH 3T3 cells. An EBV-associated nuclear antigen was detected in these cells in an indirect immunofluorescence test using anti-EBV nuclear antigen-positive human sera. These sera recognized a Mr 88,000 polypeptide in 3T3 cells transfected with the BamHI fragment K-containing polyoma virus plasmid by radioimmunoelectrophoresis. A Mr 88,000 polypeptide also was detected in a B-cell line latently infected with the B95-8 strain of EBV. Plasmids containing insertion and deletion mutations in BamHI fragment K directed the synthesis of truncated forms of the Mr 88,000 polypeptide in 3T3 cells. These data directly demonstrate that the polypeptide identified in EBV-infected lymphocyte lines by anti-EBV nuclear antigen-positive human sera is encoded by the viral genome.
将爱泼斯坦 - 巴尔病毒（EBV）BamHI限制性内切酶片段K（B95 - 8株）引入多瘤病毒表达载体，并用于转染小鼠NIH 3T3细胞。在使用抗EBV核抗原阳性人血清的间接免疫荧光试验中，在这些细胞中检测到一种EBV相关核抗原。通过放射免疫电泳，这些血清在转染了含BamHI片段K的多瘤病毒质粒的3T3细胞中识别出一种分子量为88,000的多肽。在一个被EBV B95 - 8株潜伏感染的B细胞系中也检测到了分子量为88,000的多肽。在BamHI片段K中含有插入和缺失突变的质粒指导了3T3细胞中截短形式的分子量为88,000的多肽的合成。这些数据直接证明，抗EBV核抗原阳性人血清在EBV感染的淋巴细胞系中鉴定出的多肽是由病毒基因组编码的。
