Hattori S, Kiyokawa T, Imagawa K, Shimizu F, Hashimura E, Seiki M, Yoshida M
Virology. 1984 Jul 30;136(2):338-47. doi: 10.1016/0042-6822(84)90170-3.
The gag and env gene products of human T-cell leukemia virus (HTLV) were identified with rabbit antisera against the synthetic peptides and a polypeptide produced in Escherichia coli, which corresponded to parts of the proteins predicted from the nucleotide sequence of HTLV [M. Seiki, S. Hattori, Y. Hirayama, and M. Yoshida (1983). Proc. Natl. Acad. Sci. USA 80, 3618-3622]. Viral proteins were detected by immunoprecipitation in two HTLV-producing cell lines. The precursor of gag products was a protein with an apparent molecular weight of 53,000 (Pr53), and was shown to be processed into three mature gag proteins, p19, p24, and p15, in this order, from the 5' end of the gag gene. The processing sites were confirmed to be the same as those predicted from the nucleotide sequence. The env gene product was identified as a glycoprotein of 62,000 Da (gp62), which was processed into gp46 and p20E. All the viral antigens described above were also detected with sera from ATL patients, indicating that all these proteins are expressed in the patients.
用人T细胞白血病病毒（HTLV）的gag和env基因产物，与针对合成肽和在大肠杆菌中产生的一种多肽的兔抗血清进行鉴定，该多肽对应于根据HTLV核苷酸序列预测的蛋白质部分[M. 关木、S. 服部、Y. 平山和M. 吉田（1983年）。美国国家科学院院刊80，3618 - 3622]。通过免疫沉淀在两种产生HTLV的细胞系中检测病毒蛋白。gag产物的前体是一种表观分子量为53,000的蛋白质（Pr53），并显示从gag基因的5'端开始依次加工成三种成熟的gag蛋白，p19、p24和p15。加工位点经证实与从核苷酸序列预测的位点相同。env基因产物被鉴定为一种62,000 Da的糖蛋白（gp62），它被加工成gp46和p20E。上述所有病毒抗原也能用成人T细胞白血病（ATL）患者的血清检测到，表明所有这些蛋白质在患者体内都有表达。
