Mantyh P W, Hunt S P, Maggio J E
Brain Res. 1984 Jul 30;307(1-2):147-65. doi: 10.1016/0006-8993(84)90470-0.
Substance P (SP) is a putative neurotransmitter in both the peripheral and central nervous systems. In the present report we have used a modification of the Young and Kuhar technique to investigate some of the SP receptors binding properties and the distribution of SP receptors in rat brain. Tritiated SP [( 3H]SP) absorbed extensively to glass but this adsorbtion was greatly reduced by preincubating the slide-mounted tissue sections in a solution containing the cationic polymer polyethylenimine. [3H]SP was found to bind to rat tissue in a saturable fashion with a Bmax of 14.7 fmol/mg tissue wet weight and a Kd of 1.1 nM. The rank order of potencies for displacing [3H]SP binding from rat tissue sections was SP greater than SP sulphoxide greater than DiMeC7 greater than Eledoisin greater than SP(5-11) greater than SP(COOH) greater than SP(1-9) amide. Using autoradiography coupled with LKB tritium-sensitive Ultrofilm or the dry emulsion-coated coverslip technique the distribution of [3H]SP binding sites was found to be very dense within olfactory bulb, amygdalo-hippocampal area and the nucleus of the solitary tract. Heavy concentrations of receptors were observed in the septum, diagonal band of Broca, striatum subiculum, hypothalamus, locus coeruleus, parabrachial nucleus and lobule 9 and 10 of the cerebellum. Moderate to low concentrations of receptors were observed in the cerebral cortex, globus pallidus, raphe nuclei and the trigeminal nucleus. Very low densities were observed in most aspects of the dorsal thalamus, substantia nigra and cerebellum (other than lobule 9 and 10). Comparisons of the present data with SP peptide levels indicate that in some areas of the brain there is a rough correlation between peptide and receptor levels. However, in other brain areas (olfactory bulb, globus pallidus and substantia nigra) there is little obvious correlation between the two.
P物质（SP）被认为是外周和中枢神经系统中的一种神经递质。在本报告中，我们采用了对扬和库哈尔技术的一种改进方法，来研究大鼠脑中一些SP受体的结合特性以及SP受体的分布情况。氚标记的SP[³H]SP能大量吸附在玻璃上，但通过将载玻片上的组织切片在含有阳离子聚合物聚乙烯亚胺的溶液中预孵育，这种吸附作用会大大降低。发现[³H]SP以可饱和的方式与大鼠组织结合，其最大结合容量（Bmax）为14.7 fmol/mg组织湿重，解离常数（Kd）为1.1 nM。从大鼠组织切片上取代[³H]SP结合的效力顺序为：SP＞亚砜化SP＞二甲基辛肽＞依地多辛＞SP（5 - 11）＞SP（COOH）＞SP（1 - 9）酰胺。运用放射自显影技术结合LKB对氚敏感的超薄膜或干乳剂包被盖玻片技术，发现[³H]SP结合位点在嗅球、杏仁核 - 海马区和孤束核内分布非常密集。在隔区、布罗卡斜带、纹状体下托、下丘脑、蓝斑、臂旁核以及小脑的第9和第10小叶中观察到大量受体。在大脑皮质、苍白球、中缝核和三叉神经核中观察到中度至低度的受体浓度。在背侧丘脑、黑质和小脑的大多数区域（第9和第10小叶除外）观察到极低的密度。将目前的数据与SP肽水平进行比较表明，在大脑的某些区域，肽水平和受体水平之间存在大致的相关性。然而，在其他脑区（嗅球、苍白球和黑质），两者之间几乎没有明显的相关性。
