Jordan D, Kermadi I, Rambaud C, Gilly R, Bouvier R, Kopp N
Laboratoire Anatomie Pathologique, Faculté de Médicine A. Carrel, Lyon, France.
Brain Res. 1995 Oct 16;695(2):117-24. doi: 10.1016/0006-8993(95)00632-z.
The distribution of [3H]substance P ([3H]SP) binding sites in the brainstem of the human newborn was investigated in eleven cases (aged 1 h to 6 months) by in vitro quantitative receptor autoradiography. The binding of [3H]SP to newborn brainstem tissue was found to be saturable (for the eight cases examined, Kd and Bmax (M +/- S.E.M.) were 0.29 +/- 0.03 nM and 206 +/- 21 fmol/mg tissue, respectively). Competition studies showed unlabeled SP to be the most potent peptide for displacing [3H]SP binding from tissue sections. The desaturating effect of GTP on the specific binding of [3H]SP was also investigated, but was not found to be significant. Autoradiographic analysis showed that the neurokinin-1 (NK-1)/SP binding sites were widely but unevenly distributed, and that they varied with age. The highest densities of (NK-1)/SP binding sites were observed in the locus coeruleus, olivaris inferior nuclei, raphe magnus and obscurus nuclei, while low to moderate densities were observed in other brainstem structures. These findings support the idea that SP is involved in cardiovascular regulation, and that it may interact with the catecholaminergic and/or serotonergic system.
通过体外定量受体放射自显影术，对11例（年龄从1小时至6个月）人类新生儿脑干中[3H]P物质（[3H]SP）结合位点的分布进行了研究。发现[3H]SP与新生儿脑干组织的结合具有饱和性（在所检测的8例中，Kd和Bmax（平均值±标准误）分别为0.29±0.03 nM和206±21 fmol/mg组织）。竞争研究表明，未标记的SP是从组织切片上取代[3H]SP结合的最有效肽。还研究了GTP对[3H]SP特异性结合的去饱和作用，但未发现其具有显著性。放射自显影分析显示，神经激肽-1（NK-1）/SP结合位点分布广泛但不均匀，且随年龄而变化。在蓝斑、下橄榄核、中缝大核和中缝隐核中观察到最高密度的（NK-1）/SP结合位点，而在其他脑干结构中观察到低密度至中等密度。这些发现支持了SP参与心血管调节以及它可能与儿茶酚胺能和/或5-羟色胺能系统相互作用的观点。
