Milstein C P, Deverson E V, Rabbitts T H
Nucleic Acids Res. 1984 Aug 24;12(16):6523-35. doi: 10.1093/nar/12.16.6523.
We present the full sequence of an insert of a lambda phage clone which contains a segment of human DNA stretching from the secreted mu(mu s) constant region gene through to the beginning of the constant region gene and including the membrane mu(mu m) segments. The segment of 8.6kb extending from mu s to the first constant domain of delta(C delta 1) has been completely sequenced and reveals little conservation in comparison to the corresponding mouse sequence. The outstanding feature of the mu s-mu m intron is the occurrence of a potential Z-DNA forming region situated at 285bp downstream of the mu s poly A addition signal. A similar DNA stretch exists in mouse and may represent a site for transcriptional control of mu gene expression. The mu m-C delta 1 intron is much longer (6Kb) than the corresponding mouse intron and includes a series of different repeats, which start at 430bp downstream of the mu m poly A addition site and continue for 3.5Kb, ending about 1.5Kb from the beginning of C delta 1. This series of repeats may be a vestigial switch sequence used in the production of the secreting cells which are the progenitors of the rare human IgD myelomas.
我们展示了一个λ噬菌体克隆插入片段的完整序列，该片段包含一段人类DNA，从分泌型μ（μm）恒定区基因延伸至恒定区基因起始处，并包括膜结合型μ（μm）片段。从μm到δ（Cδ1）第一个恒定结构域的8.6kb片段已被完全测序，与相应的小鼠序列相比，其保守性较低。μm-μm内含子的突出特征是在μm多聚腺苷酸添加信号下游285bp处存在一个潜在的Z-DNA形成区域。小鼠中也存在类似的DNA片段，可能代表μ基因表达的转录控制位点。μm-Cδ1内含子比相应的小鼠内含子长得多（6Kb），并包含一系列不同的重复序列，这些重复序列从μm多聚腺苷酸添加位点下游430bp处开始，持续3.5Kb，在距Cδ1起始约1.5Kb处结束。这一系列重复序列可能是用于产生分泌细胞的残余开关序列，这些分泌细胞是罕见的人类IgD骨髓瘤的祖细胞。
