• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

细菌中DNA复制的遗传分析：鼠伤寒沙门氏菌中抑制dnaC突变的dnaB突变以及抑制dnaE突变的dnaQ突变

Genetic analysis of DNA replication in bacteria: dnaB mutations that suppress dnaC mutations and dnaQ mutations that suppress dnaE mutations in Salmonella typhimurium.

作者信息

Maurer R, Osmond B C, Botstein D

出版信息

Genetics. 1984 Sep;108(1):25-38. doi: 10.1093/genetics/108.1.25.

DOI:10.1093/genetics/108.1.25
PMID:6090269
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1202398/
Abstract

We have isolated and characterized extragenic suppressors of mutations in two different target genes that affect DNA replication in Salmonella typhimurium. Both the target and the suppressor genes are functional homologues of known replication genes of E. coli that were identified in intergeneric complementation tests. Our results point to interactions in vivo involving the dnaB and dnaC proteins in one case and the dnaQ and dnaE proteins in the other case. The suppressor mutations, which were isolated as derivatives of lambda-Salmonella in vitro recombinants, were detected by an adaptation of the red plaque complementation assay. This method was applicable even when the locus of suppressor mutations was not chosen in advance.

摘要

我们已经分离并鉴定了影响鼠伤寒沙门氏菌DNA复制的两个不同靶基因的基因外抑制子。靶基因和抑制子基因都是大肠杆菌已知复制基因的功能同源物，这些同源物是在属间互补试验中鉴定出来的。我们的结果表明，在一种情况下，体内存在涉及dnaB和dnaC蛋白的相互作用；在另一种情况下，存在涉及dnaQ和dnaE蛋白的相互作用。这些抑制子突变是作为λ-沙门氏菌体外重组体的衍生物分离出来的，通过改进的红斑互补试验进行检测。即使抑制子突变的位点没有预先选定，这种方法也适用。

相似文献

1
Genetic analysis of DNA replication in bacteria: dnaB mutations that suppress dnaC mutations and dnaQ mutations that suppress dnaE mutations in Salmonella typhimurium.细菌中DNA复制的遗传分析：鼠伤寒沙门氏菌中抑制dnaC突变的dnaB突变以及抑制dnaE突变的dnaQ突变
Genetics. 1984 Sep;108(1):25-38. doi: 10.1093/genetics/108.1.25.
2
Functional interchangeability of DNA replication genes in Salmonella typhimurium and Escherichia coli demonstrated by a general complementation procedure.通过通用互补程序证明鼠伤寒沙门氏菌和大肠杆菌中DNA复制基因的功能互换性。
Genetics. 1984 Sep;108(1):1-23. doi: 10.1093/genetics/108.1.1.
3
Isolation and characterization of mutants with deletions in dnaQ, the gene for the editing subunit of DNA polymerase III in Salmonella typhimurium.鼠伤寒沙门氏菌中DNA聚合酶III编辑亚基基因dnaQ缺失突变体的分离与鉴定。
J Bacteriol. 1989 Oct;171(10):5572-80. doi: 10.1128/jb.171.10.5572-5580.1989.
4
Interactions of DNA replication factors in vivo as detected by introduction of suppressor alleles of dnaA into other temperature-sensitive dna mutants.通过将dnaA的抑制基因等位基因导入其他温度敏感型dna突变体来检测体内DNA复制因子的相互作用。
J Bacteriol. 1983 Jan;153(1):535-8. doi: 10.1128/jb.153.1.535-538.1983.
5
Cloning of bacterial DNA replication genes in bacteriophage lambda.细菌DNA复制基因在噬菌体λ中的克隆
Mol Gen Genet. 1982;187(3):501-9. doi: 10.1007/BF00332635.
6
Nucleotide sequences of dnaE, the gene for the polymerase subunit of DNA polymerase III in Salmonella typhimurium, and a variant that facilitates growth in the absence of another polymerase subunit.鼠伤寒沙门氏菌中DNA聚合酶III聚合酶亚基基因dnaE的核苷酸序列，以及一种在缺乏另一种聚合酶亚基时促进生长的变体。
J Bacteriol. 1989 Oct;171(10):5581-6. doi: 10.1128/jb.171.10.5581-5586.1989.
7
Host virus interactions in the initiation of bacteriophage lambda DNA replication. Recruitment of Escherichia coli DnaB helicase by lambda P replication protein.噬菌体λ DNA复制起始过程中的宿主-病毒相互作用。λ P复制蛋白对大肠杆菌DnaB解旋酶的招募。
J Biol Chem. 1990 Aug 5;265(22):13297-307.
8
Suppressors of recB mutations in Salmonella typhimurium.鼠伤寒沙门氏菌recB突变的抑制因子。
Genetics. 1994 Sep;138(1):11-28. doi: 10.1093/genetics/138.1.11.
9
The Escherichia coli dnaC gene product. I. Overlapping of the dnaC proteins of Escherichia coli and Salmonella typhimurium by cloning into a high copy number plasmid.大肠杆菌dnaC基因产物。I. 通过克隆到高拷贝数质粒中对大肠杆菌和鼠伤寒沙门氏菌的dnaC蛋白进行重叠分析。
J Biol Chem. 1982 Nov 25;257(22):13757-62.
10
Suppression of the DnaA phenotype by mutations in the rpoB cistron of ribonucleic acid polymerase in Salmonella typhimurium and Escherichia coli.鼠伤寒沙门氏菌和大肠杆菌中核糖核酸聚合酶rpoB顺反子突变对DnaA表型的抑制作用。
J Bacteriol. 1977 May;130(2):577-82. doi: 10.1128/jb.130.2.577-582.1977.

引用本文的文献

1
Eric Lander and David Botstein on Mapping Quantitative Traits.埃里克·兰德和大卫·博特斯坦论数量性状定位
Genetics. 2016 May;203(1):1-3. doi: 10.1534/genetics.116.189803. Epub 2016 May 5.
2
Functional interchangeability of DNA replication genes in Salmonella typhimurium and Escherichia coli demonstrated by a general complementation procedure.通过通用互补程序证明鼠伤寒沙门氏菌和大肠杆菌中DNA复制基因的功能互换性。
Genetics. 1984 Sep;108(1):1-23. doi: 10.1093/genetics/108.1.1.
3
Isolation and characterization of a photorepair-deficient mutant in Drosophila melanogaster.黑腹果蝇中光修复缺陷突变体的分离与鉴定。
Genetics. 1987 Jun;116(2):233-9. doi: 10.1093/genetics/116.2.233.
4
Linkage map of Salmonella typhimurium, edition VII.鼠伤寒沙门氏菌连锁图谱，第七版。
Microbiol Rev. 1988 Dec;52(4):485-532. doi: 10.1128/mr.52.4.485-532.1988.
5
Dominant lethal mutations in the dnaB helicase gene of Salmonella typhimurium.鼠伤寒沙门氏菌dnaB解旋酶基因中的显性致死突变。
J Bacteriol. 1988 Aug;170(8):3682-8. doi: 10.1128/jb.170.8.3682-3688.1988.
6
Sequence of the dnaB gene of Salmonella typhimurium.鼠伤寒沙门氏菌dnaB基因序列。
J Bacteriol. 1988 Jun;170(6):2668-75. doi: 10.1128/jb.170.6.2668-2675.1988.
7
Nucleotide sequences of dnaE, the gene for the polymerase subunit of DNA polymerase III in Salmonella typhimurium, and a variant that facilitates growth in the absence of another polymerase subunit.鼠伤寒沙门氏菌中DNA聚合酶III聚合酶亚基基因dnaE的核苷酸序列，以及一种在缺乏另一种聚合酶亚基时促进生长的变体。
J Bacteriol. 1989 Oct;171(10):5581-6. doi: 10.1128/jb.171.10.5581-5586.1989.
8
Isolation and characterization of mutants with deletions in dnaQ, the gene for the editing subunit of DNA polymerase III in Salmonella typhimurium.鼠伤寒沙门氏菌中DNA聚合酶III编辑亚基基因dnaQ缺失突变体的分离与鉴定。
J Bacteriol. 1989 Oct;171(10):5572-80. doi: 10.1128/jb.171.10.5572-5580.1989.
9
Genetic exploration of interactive domains in RNA polymerase II subunits.
Mol Cell Biol. 1990 May;10(5):1908-14. doi: 10.1128/mcb.10.5.1908-1914.1990.
10
DNA replication defect in Salmonella typhimurium mutants lacking the editing (epsilon) subunit of DNA polymerase III.鼠伤寒沙门氏菌缺乏DNA聚合酶III编辑（ε）亚基的突变体中的DNA复制缺陷
J Bacteriol. 1992 Nov;174(21):6965-73. doi: 10.1128/jb.174.21.6965-6973.1992.

本文引用的文献

1
Suppression of dnaC alleles by the dnaB analog (ban protein) of bacteriophage P1.噬菌体P1的dnaB类似物（ban蛋白）对dnaC等位基因的抑制作用。
J Bacteriol. 1981 Apr;146(1):321-4. doi: 10.1128/jb.146.1.321-324.1981.
2
Deoxyribonucleic acid initiation mutation dnaB252 is suppressed by elevated dnaC+ gene dosage.脱氧核糖核酸起始突变dnaB252可被升高的dnaC +基因剂量所抑制。
J Bacteriol. 1981 Apr;146(1):418-21. doi: 10.1128/jb.146.1.418-421.1981.
3
Functional cooperation of the dnaE and dnaN gene products in Escherichia coli.大肠杆菌中dnaE和dnaN基因产物的功能协作
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5764-7. doi: 10.1073/pnas.78.9.5764.
4
Genetic approaches to the analysis of microbial development.微生物发育分析的遗传学方法。
Annu Rev Genet. 1982;16:61-83. doi: 10.1146/annurev.ge.16.120182.000425.
5
The Escherichia coli dnaC gene product. III. Properties of the dnaB-dnaC protein complex.大肠杆菌dnaC基因产物。III. dnaB-dnaC蛋白复合物的特性。
J Biol Chem. 1982 Nov 25;257(22):13770-5.
6
The Escherichia coli dnaC gene product. I. Overlapping of the dnaC proteins of Escherichia coli and Salmonella typhimurium by cloning into a high copy number plasmid.大肠杆菌dnaC基因产物。I. 通过克隆到高拷贝数质粒中对大肠杆菌和鼠伤寒沙门氏菌的dnaC蛋白进行重叠分析。
J Biol Chem. 1982 Nov 25;257(22):13757-62.
7
Detection of the catalytic activities of DNA polymerases and their associated exonucleases following SDS-polyacrylamide gel electrophoresis.十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后DNA聚合酶及其相关核酸外切酶催化活性的检测。
Nucleic Acids Res. 1981 Apr 24;9(8):1825-39. doi: 10.1093/nar/9.8.1825.
8
Isolation of a complex between the P protein of phage lambda and the dnaB protein of Escherichia coli.噬菌体λ的P蛋白与大肠杆菌的dnaB蛋白之间复合物的分离。
Eur J Biochem. 1980 Mar;105(1):1-6. doi: 10.1111/j.1432-1033.1980.tb04467.x.
9
Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.对大肠杆菌中对DNA合成温度敏感的突变体的DNA聚合酶II和3的分析。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150-3. doi: 10.1073/pnas.68.12.3150.
10
Interaction of morphogenetic genes of bacteriophage T4.噬菌体T4形态发生基因的相互作用
J Mol Biol. 1970 Feb 14;47(3):293-306. doi: 10.1016/0022-2836(70)90303-7.