负超螺旋质粒中Z-DNA的形成对生理范围内盐浓度的微小变化敏感。
Formation of Z-DNA in negatively supercoiled plasmids is sensitive to small changes in salt concentration within the physiological range.
作者信息
Azorin F, Nordheim A, Rich A
出版信息
EMBO J. 1983;2(5):649-55. doi: 10.1002/j.1460-2075.1983.tb01479.x.
Abstract
Negative supercoiling of the plasmid pBR322 with or without an insert of (dG-dC)n induces the formation of Z-DNA as measured by the binding of antibodies specific for Z-DNA. Increasing the concentration of Na+ (or K+) is shown to inhibit the B to Z-DNA conversion. This may be due to the effect of the cation on the B-Z junction. Using the data for B to Z-DNA conversion of the (dG-dC)n inserts, we have estimated the free energy change per base pair as well as the energy of the B-Z junction. In pBR322, a 14-bp segment [CACGGGTGCGCATG] is believed to form Z-DNA at bacterial negative superhelical densities under salt conditions which are similar to those found in vivo.
摘要
无论有无(dG-dC)n插入片段，质粒pBR322的负超螺旋都会诱导Z-DNA的形成，这可通过Z-DNA特异性抗体的结合来测定。结果表明，增加Na+（或K+）浓度可抑制B-DNA向Z-DNA的转变。这可能是由于阳离子对B-Z连接处的影响。利用(dG-dC)n插入片段从B-DNA向Z-DNA转变的数据，我们估算了每个碱基对的自由能变化以及B-Z连接处的能量。在pBR322中，一个14个碱基对的片段[CACGGGTGCGCATG]被认为在类似于体内发现的盐条件下，于细菌负超螺旋密度时形成Z-DNA。
相似文献
1
Formation of Z-DNA in negatively supercoiled plasmids is sensitive to small changes in salt concentration within the physiological range.负超螺旋质粒中Z-DNA的形成对生理范围内盐浓度的微小变化敏感。
EMBO J. 1983;2(5):649-55. doi: 10.1002/j.1460-2075.1983.tb01479.x.
2
The effect of anti-Z-DNA antibodies on the B-DNA-Z-DNA equilibrium.抗Z-DNA抗体对B-DNA-Z-DNA平衡的影响。
J Biol Chem. 1986 May 15;261(14):6438-43.
3
Negatively supercoiled plasmids contain left-handed Z-DNA segments as detected by specific antibody binding.通过特异性抗体结合检测发现，负超螺旋质粒含有左手Z-DNA片段。
Cell. 1982 Dec;31(2 Pt 1):309-18. doi: 10.1016/0092-8674(82)90124-6.
4
Topoisomerase mutants and physiological conditions control supercoiling and Z-DNA formation in vivo.拓扑异构酶突变体和生理条件在体内控制超螺旋和Z-DNA形成。
J Biol Chem. 1991 Feb 5;266(4):2576-81.
5
Left-handed Z-DNA helices in polymers, restriction fragments, and recombinant plasmids.聚合物、限制性片段和重组质粒中的左手Z-DNA螺旋。
J Biomol Struct Dyn. 1983 Dec;1(4):999-1009. doi: 10.1080/07391102.1983.10507498.
6
7
Effects of 5 cytosine methylation on the B-Z transition in DNA restriction fragments and recombinant plasmids.5-胞嘧啶甲基化对DNA限制性片段和重组质粒中B-Z转变的影响。
J Mol Biol. 1983 Jul 25;168(1):51-71. doi: 10.1016/s0022-2836(83)80322-2.
8
The sequence (dC-dA)n X (dG-dT)n forms left-handed Z-DNA in negatively supercoiled plasmids.序列（dC-dA）n×（dG-dT）n在负超螺旋质粒中形成左手Z-DNA。
Proc Natl Acad Sci U S A. 1983 Apr;80(7):1821-5. doi: 10.1073/pnas.80.7.1821.
9
Interaction in vitro of type III intermediate filament proteins with supercoiled plasmid DNA and modulation of eukaryotic DNA topoisomerase I and II activities.III型中间丝蛋白与超螺旋质粒DNA的体外相互作用以及真核DNA拓扑异构酶I和II活性的调节
DNA Cell Biol. 2002 Oct;21(10):743-69. doi: 10.1089/104454902760599726.
10
An assessment of the Z-DNA forming potential of alternating dA-dT stretches in supercoiled plasmids.超螺旋质粒中交替dA-dT片段形成Z-DNA潜力的评估。
Biochemistry. 1986 Jun 17;25(12):3648-55. doi: 10.1021/bi00360a026.
引用本文的文献
1
DNA supercoiling-mediated G4/R-loop formation tunes transcription by controlling the access of RNA polymerase.DNA超螺旋介导的G4/R环形成通过控制RNA聚合酶的进入来调节转录。
Nat Commun. 2025 Apr 9;16(1):3363. doi: 10.1038/s41467-025-58479-x.
2
Z-form DNA-RNA hybrid blocks DNA replication.Z型DNA-RNA杂交体阻断DNA复制。
Nucleic Acids Res. 2025 Feb 27;53(5). doi: 10.1093/nar/gkaf135.
3
The role of DNA in the pathogenesis of SLE: DNA as a molecular chameleon.DNA 在 SLE 发病机制中的作用：DNA 作为分子变色龙。
Ann Rheum Dis. 2024 Jun 12;83(7):830-837. doi: 10.1136/ard-2023-225266.
4
The Binding Properties of Antibodies to Z-DNA in the Sera of Normal Healthy Subjects.正常健康受试者血清中抗体与Z-DNA的结合特性。
Int J Mol Sci. 2024 Feb 22;25(5):2556. doi: 10.3390/ijms25052556.
5
Creation and resolution of non-B-DNA structural impediments during replication.复制过程中非 B-DNA 结构障碍的形成与解决。
Crit Rev Biochem Mol Biol. 2022 Aug;57(4):412-442. doi: 10.1080/10409238.2022.2121803. Epub 2022 Sep 28.
6
Impact of G-Quadruplexes on the Regulation of Genome Integrity, DNA Damage and Repair.G-四链体对基因组完整性、DNA 损伤与修复调控的影响
Biomolecules. 2021 Aug 27;11(9):1284. doi: 10.3390/biom11091284.
7
The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin.单克隆和多克隆抗 Z-DNA 抗体与各种来源 DNA 的结合。
Int J Mol Sci. 2021 Aug 19;22(16):8931. doi: 10.3390/ijms22168931.
8
G-quadruplex-R-loop interactions and the mechanism of anticancer G-quadruplex binders.G-四链体-R 环相互作用与抗癌 G-四链体结合物的作用机制。
Nucleic Acids Res. 2020 Dec 2;48(21):11942-11957. doi: 10.1093/nar/gkaa944.
9
The effects of DNA supercoiling on G-quadruplex formation.DNA超螺旋对G-四链体形成的影响。
Nucleic Acids Res. 2017 Dec 1;45(21):12069-12079. doi: 10.1093/nar/gkx856.
10
NMR Studies of an SV40 Enhancer Core DNA Sequence.猴空泡病毒40增强子核心DNA序列的核磁共振研究
Biophys J. 1986 Jan;49(1):26-9. doi: 10.1016/S0006-3495(86)83580-9.
本文引用的文献
1
The high salt form of poly(dG-dC).poly(dG-dC) is left-handed Z-DNA: Raman spectra of crystals and solutions.聚（dG-dC）·聚（dG-dC）的高盐形式是左手Z-DNA：晶体和溶液的拉曼光谱。
Nucleic Acids Res. 1981 Oct 24;9(20):5443-57. doi: 10.1093/nar/9.20.5443.
2
Mn2+ and other transition metals at low concentration induce the right-to-left helical transformation of poly[d(G-C)].低浓度的锰离子（Mn2+）和其他过渡金属会诱导聚[d(G-C)]从右向左的螺旋转变。
EMBO J. 1982;1(7):777-82. doi: 10.1002/j.1460-2075.1982.tb01247.x.
3
Negatively supercoiled plasmids contain left-handed Z-DNA segments as detected by specific antibody binding.通过特异性抗体结合检测发现，负超螺旋质粒含有左手Z-DNA片段。
Cell. 1982 Dec;31(2 Pt 1):309-18. doi: 10.1016/0092-8674(82)90124-6.
4
High magnesium content of Escherichia coli B.
Experientia. 1981;37(8):846-7. doi: 10.1007/BF01985672.
5
Flipping of cloned d(pCpG)n.d(pCpG)n DNA sequences from right- to left-handed helical structure by salt, Co(III), or negative supercoiling.通过盐、钴（III）或负超螺旋使克隆的d(pCpG)n.d(pCpG)n DNA序列从右手螺旋结构转变为左手螺旋结构。
Proc Natl Acad Sci U S A. 1982 Aug;79(15):4560-4. doi: 10.1073/pnas.79.15.4560.
6
Antibodies specific for left-handed Z-DNA.对左手Z-DNA具有特异性的抗体。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3546-50. doi: 10.1073/pnas.78.6.3546.
7
Antibodies to left-handed Z-DNA bind to interband regions of Drosophila polytene chromosomes.抗左旋Z-DNA的抗体与果蝇多线染色体的间带区域结合。
Nature. 1981 Dec 3;294(5840):417-22. doi: 10.1038/294417a0.
8
Isolation of Drosophila proteins that bind selectively to left-handed Z-DNA.果蝇中选择性结合左旋Z-DNA的蛋白质的分离。
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7729-33. doi: 10.1073/pnas.79.24.7729.
9
Left-handed Z-DNA is induced by supercoiling in physiological ionic conditions.在生理离子条件下，超螺旋可诱导左手Z-DNA的形成。
Nature. 1982 Sep 23;299(5881):312-6. doi: 10.1038/299312a0.
10
Effects of methylation on a synthetic polynucleotide: the B--Z transition in poly(dG-m5dC).poly(dG-m5dC).甲基化对合成多核苷酸的影响：聚（dG-m5dC）·聚（dG-m5dC）中的B-Z转变
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1619-23. doi: 10.1073/pnas.78.3.1619.
Zotero 插件