Hosaka K, Yamashita S
Biochim Biophys Acta. 1984 Oct 24;796(1):110-7.
The regulatory mechanism of triacylglycerol synthesis in Saccharomyces cerevisiae was studied. The triacylglycerol content increased markedly during the entry of cells into the stationary growth phase, whereas the content of phospholipids remained unchanged. Pulse-labeling experiments to measure [14C]acetate incorporation into triacylglycerol revealed that the synthesis of triacylglycerol increased in the stationary growth phase. An increase in fatty acid synthesis was observed only in the later stage of the stationary growth phase and thus does not seem to be the principal causative factor for the triacylglycerol accumulation. Among various triacylglycerol-synthetic enzymes tested, the increase in the phosphatidate phosphatase (EC 3.1.3.4) activity was most closely correlated with the accumulation of triacylglycerol in the stationary phase. Our results show that phosphatidate phosphatase plays an important role in the regulation of triacylglycerol synthesis in S. cerevisiae.
对酿酒酵母中三酰甘油合成的调控机制进行了研究。在细胞进入稳定生长期时，三酰甘油含量显著增加，而磷脂含量保持不变。用于测量[14C]乙酸掺入三酰甘油的脉冲标记实验表明，在稳定生长期三酰甘油的合成增加。仅在稳定生长期后期观察到脂肪酸合成增加，因此似乎不是三酰甘油积累的主要致病因素。在测试的各种三酰甘油合成酶中，磷脂酸磷酸酶（EC 3.1.3.4）活性的增加与稳定期三酰甘油的积累最密切相关。我们的结果表明，磷脂酸磷酸酶在酿酒酵母三酰甘油合成的调控中起重要作用。
