Brown N, Smith D, Miller G, Niederman J, Liu C, Robinson J
J Infect Dis. 1984 Oct;150(4):517-22. doi: 10.1093/infdis/150.4.517.
We obtained spontaneous formation of Epstein-Barr virus-transformed B lymphocyte colonies from the blood of four patients with mononucleosis with the use of soft-agar medium. The colonies were propagated into separate cell lines and analyzed for their immunoglobulin secretion. Of 52 such lines, 44 produced immunoglobulin composed of a single class of heavy chain and single type of light chain. Among these clonal transformants, 27 (62%) of 44 produced mu-chain, 13 (29%) of 44 produced gamma-chain, and 4 (9%) of 44 produced alpha-chain. Analysis of light-chain secretion revealed that of the 44 cell clones secreting complete, monoclonal immunoglobulin products, 31 produced kappa-chain and 13 produced gamma-chain. One clone secreted mu-heavy chain and no light chain, an observation suggesting the clone is a pre-B cell phenotype. Seven lines derived from clones had aberrant patterns of immunoglobulin secretion that produced either two heavy chains or two light chains. B lymphocytes in different states of immunoglobulin gene expression are, therefore, transformed in vivo by Epstein-Barr virus.
我们使用软琼脂培养基从4例单核细胞增多症患者的血液中获得了爱泼斯坦-巴尔病毒转化的B淋巴细胞集落。这些集落被传代培养成单独的细胞系，并对其免疫球蛋白分泌情况进行了分析。在52个这样的细胞系中，44个产生了由单一重链类别和单一轻链类型组成的免疫球蛋白。在这些克隆转化体中，44个中有27个（62%）产生μ链，44个中有13个（29%）产生γ链，44个中有4个（9%）产生α链。轻链分泌分析显示，在44个分泌完整单克隆免疫球蛋白产物的细胞克隆中，31个产生κ链，13个产生γ链。一个克隆分泌μ重链但不分泌轻链，这一观察结果表明该克隆是前B细胞表型。来自克隆的7个细胞系具有异常的免疫球蛋白分泌模式，产生两条重链或两条轻链。因此，处于免疫球蛋白基因表达不同状态的B淋巴细胞在体内被爱泼斯坦-巴尔病毒转化。
