Perez-Schael I, Daoud G, White L, Urbina G, Daoud N, Perez M, Flores J
J Med Virol. 1984;14(2):127-36. doi: 10.1002/jmv.1890140206.
We studied the shedding of rotavirus by newborn children in the nurseries of a large maternity hospital in Caracas, Venezuela, throughout the year 1982. Sixty-two (57%) of 108 children examined shed the virus within the first few days of life. Four (6%) of the 62 children who shed rotavirus had diarrhea but only one of them required oral rehydration therapy. The rotavirus specimens were identified as subgroup 2 in an ELISA subgrouping assay that employs monoclonal antibodies. Analysis of the RNA extracted from 52 of the samples by electrophoresis revealed a similar migration pattern in all the specimens; their identity was confirmed by crosshybridization analyses which revealed a strong degree of genomic homology among the strains studied.
1982年全年，我们对委内瑞拉加拉加斯一家大型妇产医院托儿所内的新生儿轮状病毒排出情况进行了研究。在接受检查的108名儿童中，有62名（57%）在出生后的头几天内排出了该病毒。排出轮状病毒的62名儿童中有4名（6%）出现腹泻，但其中只有1名需要口服补液治疗。在一项采用单克隆抗体的ELISA亚组分析中，轮状病毒标本被鉴定为2亚组。通过电泳对52个样本提取的RNA进行分析，结果显示所有标本的迁移模式相似；通过交叉杂交分析证实了它们的一致性，该分析表明所研究的毒株之间存在高度的基因组同源性。
