Mendoza M C, Blanco M G, Javier Mendez F, Hardisson C
Pathol Biol (Paris). 1984 Sep;32(7):750-4.
A study on the evolution of resistance to six aminoglycosides in Serratia as well as the relationship with the annual consumption of each drug in a hospital over the period 1974-1981 was carried out. The incidence of modifying enzymes and their genetical location was determined in 38 isolates. It was found that: The variations in the percentage of Sm clinical isolates showed no relationship with the consumption of Sm. Two different types of enzymes are involved in this resistance: AAD(3''): adenylyltransferase and APH(3''): phosphotransferase. The resistance to Nm, Km and Gm seems to be directly related with the continuous consumption of these drugs. In all the strains under study (Nm-Km)r was due to an APH(3')(5)I: phosphotransferase, Gmr to two types of acetyltransferases: AAC(3)I, only found in strains isolated before 1977 and AAC(3)II which predominates in 1981. After introducing Tm (1975) and Ak (1979) in our environment, there was an increase in the number of resistant strains. Two acetyltransferases with Tm-affinity were found: AAC(3)II and AAC(6')IV, the latter showing affinity for AKr. It was determined that five of these enzymes are plasmid-mediated. The genetical location of a sixth enzyme (AAC(6')IV) has not been clarified.
开展了一项关于沙雷氏菌对六种氨基糖苷类抗生素耐药性演变以及与1974 - 1981年期间某医院每种药物年消耗量之间关系的研究。测定了38株分离菌中修饰酶的发生率及其基因定位。结果发现：庆大霉素临床分离株百分比的变化与庆大霉素的消耗量无关。这种耐药性涉及两种不同类型的酶：AAD(3'')：腺苷酸转移酶和APH(3'')：磷酸转移酶。对新霉素、卡那霉素和庆大霉素的耐药性似乎与这些药物的持续使用直接相关。在所有研究菌株中，(新霉素 - 卡那霉素)耐药是由于APH(3')(5)I：磷酸转移酶，庆大霉素耐药是由于两种类型的乙酰转移酶：AAC(3)I，仅在1977年以前分离的菌株中发现，以及AAC(3)II，在1981年占主导地位。在我们的环境中引入妥布霉素(1975年)和阿贝卡星(1979年)后，耐药菌株数量增加。发现了两种对妥布霉素有亲和力的乙酰转移酶：AAC(3)II和AAC(6')IV，后者对阿贝卡星耐药有亲和力。确定其中五种酶是质粒介导的。第六种酶(AAC(6')IV)的基因定位尚未明确。
