Terán F J, Suárez J E, Mendoza M C
Departamento de Biología Funcional, Universidad de Oviedo, Spain.
Antimicrob Agents Chemother. 1991 Apr;35(4):714-9. doi: 10.1128/AAC.35.4.714.
A gene coding for an aminoglycoside 6'-N-acetyltransferase that was able to modify amikacin was cloned from a plasmid isolated from a clinical strain of Enterobacter cloacae. Sequencing of a 955-bp segment which mediates the modifying activity revealed a single open reading frame of 432 nucleotides that predicted a polypeptide of 144 amino acid residues with a molecular weight of 16,021. Putative ribosomal binding sites and -10 and -35 sequences were located at the 5' end of the gene. The size of the polypeptide was confirmed through minicell analysis of the expression products of plasmids containing the sequence. The use of the gene as a molecular probe revealed its specificity toward strains harboring genes coding for related enzymes. This probe is therefore useful for epidemiological studies.
从阴沟肠杆菌临床菌株分离的质粒中克隆到一个编码能修饰阿米卡星的氨基糖苷6'-N-乙酰基转移酶的基因。对介导修饰活性的955 bp片段进行测序，发现一个432个核苷酸的单一开放阅读框，预测其编码一个由144个氨基酸残基组成、分子量为16,021的多肽。推测的核糖体结合位点以及-10和-35序列位于该基因的5'端。通过对含有该序列的质粒表达产物进行微小细胞分析，证实了该多肽的大小。将该基因用作分子探针显示了其对携带编码相关酶基因的菌株的特异性。因此，该探针可用于流行病学研究。
