Virtanen A, Aleström P, Persson H, Katze M G, Pettersson U
Nucleic Acids Res. 1982 Apr 24;10(8):2539-48. doi: 10.1093/nar/10.8.2539.
The nucleotide sequence of a 550 base pairs long segment, located between map positions 21 and 22.5 in the adenovirus type 2 genome has been determined. S1 nuclease mapping and sequence analysis of cDNA copies of adenovirus mRNA demonstrated that the established sequence includes the i-leader which is spliced to the 5'-end of certain adenovirus mRNAs (1). The i-leader which is 440 nucleotides long contains an open translational reading frame which is preceded by an AUG triplet and which terminates in the third segment of the tripartite leader. A polypeptide with the same molecular weight as predicted from the DNA sequence was identified by in vitro translation of mRNA which had been selected by hybridization to DNA fragments, containing sequences from the i-leader. The results thus suggest that the i-leader, unlike other adenovirus leader segments, is used for translation.
已确定腺病毒2型基因组中位于图谱位置21和22.5之间的一段550个碱基对长片段的核苷酸序列。对腺病毒mRNA的cDNA拷贝进行S1核酸酶图谱分析和序列分析表明，确定的序列包括与某些腺病毒mRNA 5'-末端拼接的i-前导序列（1）。440个核苷酸长的i-前导序列包含一个开放的翻译阅读框，该阅读框之前是一个AUG三联体，并在三联体前导序列的第三段终止。通过对与含有i-前导序列的DNA片段杂交选择的mRNA进行体外翻译，鉴定出一种与从DNA序列预测的分子量相同的多肽。因此，结果表明，与其他腺病毒前导序列不同，i-前导序列用于翻译。
