Akusjärvi G, Zabielski J, Perricaudet M, Pettersson U
Nucleic Acids Res. 1981 Jan 10;9(1):1-17. doi: 10.1093/nar/9.1.1.
We report the sequence of a 1164 nucleotide long DNA segment, located between map positions 59.5 and 62.8 on the adenovirus type 2 genome. The sequence comprises the 701 nucleotides long 3' non-coding region of the hexon mRNA as well as several important processing signals. The sequence revealed unexpectedly that the 3' non-coding region of the hexon mRNA contains a 609 nucleotide long uninterrupted translational reading frame following a potential initiator AUG. A late 14S mRNA, corresponding to the open reading frame, could be identified by S1 nuclease mapping and electronmicroscopy. The mRNA shares a poly(A) addition site with the hexon and pVI mRNAs, and carries a leader sequence which is related, and probably identical, to the tripartite leader, found in late adenovirus mRNAs. The junction between the leader and the body of this novel mRNA is located within the coding part of the hexon gene.
我们报告了一段1164个核苷酸长的DNA片段的序列，该片段位于腺病毒2型基因组图谱位置59.5和62.8之间。该序列包含六邻体mRNA 701个核苷酸长的3'非编码区以及几个重要的加工信号。该序列意外地显示，六邻体mRNA的3'非编码区在一个潜在的起始AUG之后包含一个609个核苷酸长的不间断翻译阅读框。通过S1核酸酶图谱分析和电子显微镜可以鉴定出与该开放阅读框相对应的晚期14S mRNA。该mRNA与六邻体和pVI mRNA共用一个聚腺苷酸化位点，并带有一个前导序列，该前导序列与晚期腺病毒mRNA中发现的三联体前导序列相关且可能相同。这种新型mRNA的前导序列与主体之间的连接处位于六邻体基因的编码部分内。
