Gordon J D, Carstens E B
Virology. 1984 Oct 15;138(1):69-81. doi: 10.1016/0042-6822(84)90148-x.
A ts mutant of Autographa californica nuclear polyhedrosis virus (AcMNPV), ts8, was shown to be defective in viral DNA synthesis at the nonpermissive temperature. Ts8-infected cells synthesized only early viral polypeptides at the nonpermissive temperature, and in contrast to wild-type (WT)-infected cells, showed no inhibition of host cell protein synthesis. The effect of the mutation on viral DNA synthesis was not immediately reversed after shifting infected cells down from the nonpermissive temperature to the permissive temperature; rather, a delay of several hours occurred before viral DNA synthesis was detected. The rate of accumulation of viral DNA in ts8-infected cells failed to increase after a shift from the permissive temperature to the nonpermissive temperature. This indicated that the ts8 mutation was involved in the synthesis of proteins required for viral DNA synthesis. The mutation was mapped by marker rescue to the region lying between 60.1 and 62.0% of the AcMNPV physical map.
苜蓿银纹夜蛾核型多角体病毒（AcMNPV）的一个温度敏感（ts）突变体ts8，已被证明在非允许温度下病毒DNA合成存在缺陷。ts8感染的细胞在非允许温度下仅合成早期病毒多肽，并且与野生型（WT）感染的细胞不同，未表现出对宿主细胞蛋白质合成的抑制。将感染细胞从非允许温度下调至允许温度后，该突变对病毒DNA合成的影响并未立即逆转；相反，在检测到病毒DNA合成之前出现了数小时的延迟。从允许温度转变为非允许温度后，ts8感染细胞中病毒DNA的积累速率未能增加。这表明ts8突变与病毒DNA合成所需蛋白质的合成有关。通过标记拯救将该突变定位到AcMNPV物理图谱60.1%至62.0%之间的区域。
