Ratmanova K I, Bocharova T N, Andreeva L A
Bioorg Khim. 1984 Apr;10(4):564-6.
Six oligodeoxyribonucleotides ranging from 9-mer to 13-mer were synthesized in solution by the phosphotriester approach and enzymatically joined by T4 DNA ligase. The obtained double-stranded DNA (32 b.p.) with protruding 5'-ends corresponding to the recognition sites for restrictases EcoRI and BamHI represents an oligonucleotide template coding for the modified amino acid sequence 4-10 of the adrenocorticotropic hormone, [Pro8,Gly9,Pro10]ACTH-(4-10).
通过磷酸三酯法在溶液中合成了6种长度从9聚体到13聚体的寡脱氧核糖核苷酸，并通过T4 DNA连接酶进行酶促连接。所获得的双链DNA（32个碱基对）具有与限制性内切酶EcoRI和BamHI识别位点相对应的突出5'末端，它代表了一种寡核苷酸模板，编码促肾上腺皮质激素的修饰氨基酸序列4 - 10，即[Pro8，Gly9，Pro10]ACTH-(4 - 10)。
