Beesley J E, Day S E, Betts M P, Thorley C M
J Gen Microbiol. 1984 Jun;130(6):1481-7. doi: 10.1099/00221287-130-6-1481.
An immunogold labelling technique was used to label the pili of the bacterium Bacteroides nodosus. The labelling was distinct and highly specific, and individual pili could be recognised beneath the gold probe. The labelling of somatic antigens could be distinguished from that of pilus antigens. Furthermore, labelling of fragments of cytoplasm released by cell lysis and trapped in the pili could be distinguished from pilus labelling. An antiserum that had been raised against strain 80200 (serotype N) labelled pili of strain 215 (serotype B). Double labelling experiments with this antiserum and the antiserum against strain 215 (serotype B) showed that both antisera label the same pili bundles. The ease of detection of the immunocytochemical reaction should enable this technique to be used as a routine screen for pilus antigens. It also possesses the potential for much wider applications for immunolabelling other antigens, such as viruses, that can be obtained in suspension.
采用免疫金标记技术对结节拟杆菌的菌毛进行标记。标记清晰且具有高度特异性，在金探针下可识别出单个菌毛。菌体抗原的标记与菌毛抗原的标记可区分开来。此外，细胞裂解释放并被困在菌毛中的细胞质片段的标记也可与菌毛标记区分开。用针对80200菌株（血清型N）制备的抗血清可标记215菌株（血清型B）的菌毛。用该抗血清与针对215菌株（血清型B）的抗血清进行双重标记实验表明，两种抗血清标记的是相同的菌毛束。免疫细胞化学反应易于检测，这使得该技术可作为菌毛抗原的常规筛选方法。它还具有更广泛的应用潜力，可用于免疫标记其他可悬浮获得的抗原，如病毒。
