Ash K O, Smith J B, Kemp J W, Lynch M B, Moody F G, Raymond J L, McKnight M R, Williams R R
Clin Physiol Biochem. 1983;1(6):293-9.
Ouabain binding to erythrocyte membranes is increased in obese subjects. Three study groups are compared: 14 reference subjects, 102 +/- 16% of ideal weight; 9 obese on unrestricted diets, 207 +/- 16% of ideal weight; 11 obese on restricted diets, 202 +/- 35% of ideal weight. A reproducible (CV = 11.3%) ouabain-binding assay is used to measure Na+-K+ ATPase sites in erythrocyte membranes. The number of binding sites per red blood cell for obese subjects on unrestricted diets, 431 +/- 30, is greater than for the reference group, 346 +/- 66 (p less than 0.01), or for obese subjects on restricted diets, 371 +/- 68 (p less than 0.05). These data suggest that caloric intake influences the number of Na+-K+ ATPase sites. Scatchard plots indicate only one type of binding site for ouabain with an affinity constant of about 3 X 10(8) M-1.
哇巴因与红细胞膜的结合在肥胖受试者中增加。比较了三个研究组：14名参考受试者，体重为理想体重的102±16%；9名饮食不受限制的肥胖者，体重为理想体重的207±16%；11名饮食受限的肥胖者，体重为理想体重的202±35%。采用一种可重复的（变异系数=11.3%）哇巴因结合测定法来测量红细胞膜中的钠钾ATP酶位点。饮食不受限制的肥胖受试者每个红细胞的结合位点数为431±30，高于参考组的346±66（p<0.01），也高于饮食受限的肥胖受试者的371±68（p<0.05）。这些数据表明热量摄入会影响钠钾ATP酶位点的数量。Scatchard图显示哇巴因只有一种结合位点，其亲和常数约为3×10⁸M⁻¹。
