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H-2Kd基因转录本5'部分的可变剪接。

Alternative splicing in the 5' moiety of the H-2Kd gene transcript.

作者信息

Transy C, Lalanne J L, Kourilsky P

出版信息

EMBO J. 1984 Oct;3(10):2383-6. doi: 10.1002/j.1460-2075.1984.tb02143.x.

DOI:10.1002/j.1460-2075.1984.tb02143.x
PMID:6094182
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC557697/
Abstract

The H-2Kd gene, which encodes a mouse major transplantation antigen, was transfected into L TK- mouse fibroblasts. Two transcripts of the gene were detected by S1 nuclease mapping analysis. They correspond to two previously characterized cDNA clones isolated from DBA/2 mouse liver RNA, leading to the conclusion that the H-2Kd gene gives rise to two distinct transcripts through an alternate use of splicing sites. The non-canonical RNA potentially encodes a so far undescribed H-2Kd-like molecule. It is present in all tissues tested (liver, spleen, thymus, kidney) albeit in lower amounts (approximately 10-fold less) than the canonical RNA coding for H-2Kd.

摘要

编码小鼠主要移植抗原的H-2Kd基因被转染到L TK-小鼠成纤维细胞中。通过S1核酸酶图谱分析检测到该基因的两种转录本。它们与从DBA/2小鼠肝脏RNA中分离出的两个先前已鉴定的cDNA克隆相对应，从而得出结论：H-2Kd基因通过剪接位点的交替使用产生两种不同的转录本。这种非规范RNA可能编码一种迄今未描述的H-2Kd样分子。它存在于所有测试组织（肝脏、脾脏、胸腺、肾脏）中，尽管其含量比编码H-2Kd的规范RNA少（约少10倍）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a2/557697/7ac37b4afb8e/emboj00314-0177-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a2/557697/b89077b7f3ae/emboj00314-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a2/557697/7ac37b4afb8e/emboj00314-0177-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a2/557697/b89077b7f3ae/emboj00314-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a2/557697/7ac37b4afb8e/emboj00314-0177-a.jpg

相似文献

1
Alternative splicing in the 5' moiety of the H-2Kd gene transcript.H-2Kd基因转录本5'部分的可变剪接。
EMBO J. 1984 Oct;3(10):2383-6. doi: 10.1002/j.1460-2075.1984.tb02143.x.
2
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3
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引用本文的文献

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Unusual RNA splicing generates a secreted form of HLA-A2 in a mutagenized B lymphoblastoid cell line.异常的RNA剪接在诱变的B淋巴母细胞系中产生一种分泌形式的HLA - A2。
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2
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本文引用的文献

1
The DNA sequence of the H-2kb gene: evidence for gene conversion as a mechanism for the generation of polymorphism in histocompatibilty antigens.H-2kb基因的DNA序列：基因转换作为组织相容性抗原多态性产生机制的证据。
EMBO J. 1983;2(3):453-62. doi: 10.1002/j.1460-2075.1983.tb01444.x.
2
Mouse histocompatibility genes: structure and organisation of a Kd gene.小鼠组织相容性基因：Kd基因的结构与组织
EMBO J. 1983;2(2):245-54. doi: 10.1002/j.1460-2075.1983.tb01413.x.
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DNA sequence of a gene encoding a BALB/c mouse Ld transplantation antigen.
J Exp Med. 1986 May 1;163(5):1173-90. doi: 10.1084/jem.163.5.1173.
4
Alternative splicing in the mouse H-2Kd gene is not necessary for the classical Kd antigen function.小鼠H-2Kd基因中的可变剪接对于经典Kd抗原功能并非必需。
Immunogenetics. 1986;24(4):267-74. doi: 10.1007/BF00364531.
5
T3 delta pre-mRNA is transcribed from a non-TATA promoter and is alternatively spliced in human T cells.T3δ前体mRNA从非TATA启动子转录而来，并在人T细胞中进行可变剪接。
EMBO J. 1986 Jun;5(6):1245-52. doi: 10.1002/j.1460-2075.1986.tb04353.x.
6
Alternative protein products with different carboxyl termini from a single class I gene, H-2Kb.来自单个 I 类基因 H-2Kb 的具有不同羧基末端的替代蛋白产物。
Proc Natl Acad Sci U S A. 1986 Aug;83(16):6084-8. doi: 10.1073/pnas.83.16.6084.
7
Primary structure of the gene for the murine Ia antigen-associated invariant chains (Ii). An alternatively spliced exon encodes a cysteine-rich domain highly homologous to a repetitive sequence of thyroglobulin.小鼠Ia抗原相关恒定链（Ii）基因的一级结构。一个选择性剪接的外显子编码一个富含半胱氨酸的结构域，该结构域与甲状腺球蛋白的一个重复序列高度同源。
EMBO J. 1987 Jun;6(6):1677-83. doi: 10.1002/j.1460-2075.1987.tb02417.x.
8
Isolation, expression, and the primary structure of HLA-Cw1 and HLA-Cw2 genes: evolutionary aspects.HLA - Cw1和HLA - Cw2基因的分离、表达及一级结构：进化方面
Immunogenetics. 1987;25(5):313-22. doi: 10.1007/BF00404424.
9
Studies on the expression of an H-2K/human growth hormone fusion gene in giant transgenic mice.H - 2K/人类生长激素融合基因在转基因巨型小鼠中的表达研究。
EMBO J. 1986 Aug;5(8):1877-83. doi: 10.1002/j.1460-2075.1986.tb04439.x.
10
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EMBO J. 1985 Dec 16;4(13A):3447-53. doi: 10.1002/j.1460-2075.1985.tb04103.x.
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4
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5
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6
Identification of the class I genes of the mouse major histocompatibility complex by DNA-mediated gene transfer.通过DNA介导的基因转移鉴定小鼠主要组织相容性复合体的I类基因。
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Alternative RNA splicing in expression of the H-2K gene.H-2K基因表达中的可变RNA剪接。
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