小鼠I类基因5'端不同的外显子-内含子结构用于产生一种新的与H-2Kd相关的mRNA。
Different exon-intron organization at the 5' part of a mouse class I gene is used to generate a novel H-2Kd-related mRNA.
作者信息
Lalanne J L, Cochet M, Kummer A M, Gachelin G, Kourilsky P
出版信息
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7561-5. doi: 10.1073/pnas.80.24.7561.
Abstract
A cDNA library constructed from liver mRNA of DBA/2 (H-2d) mice has been screened with H-2-specific probes. The nucleotide sequence of one clone (pH-2d-24) indicates that it derives from an H-2 gene with an unexpected exon-intron organization. Nucleotide sequence comparisons suggest that two distinct mRNAs are produced from a single H-2Kd gene by a mechanism involving the use of alternative splicing sites in its 5' region. pH-2d-24 carries an open reading frame encoding a thus-far-undescribed polypeptide product identical to an H-2Kd-molecule, except for the NH2-terminal half of the first domain.
摘要
用H - 2特异性探针筛选了由DBA/2（H - 2d）小鼠肝脏mRNA构建的cDNA文库。一个克隆（pH - 2d - 24）的核苷酸序列表明它源自一个具有意外外显子 - 内含子组织的H - 2基因。核苷酸序列比较表明，通过一种涉及在其5'区域使用可变剪接位点的机制，从单个H - 2Kd基因产生了两种不同的mRNA。pH - 2d - 24携带一个开放阅读框，该阅读框编码一种迄今为止未描述的多肽产物，该产物与H - 2Kd分子相同，除了第一个结构域的NH2末端一半。
相似文献
1
Different exon-intron organization at the 5' part of a mouse class I gene is used to generate a novel H-2Kd-related mRNA.小鼠I类基因5'端不同的外显子-内含子结构用于产生一种新的与H-2Kd相关的mRNA。
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7561-5. doi: 10.1073/pnas.80.24.7561.
2
Expression of class I genes in the major histocompatibility complex: identification of eight distinct mRNAs in DBA/2 mouse liver.主要组织相容性复合体中I类基因的表达：在DBA/2小鼠肝脏中鉴定出八种不同的mRNA。
Cell. 1985 Jun;41(2):469-78. doi: 10.1016/s0092-8674(85)80020-9.
3
Identification of an H-2Kd gene using a specific cDNA probe.使用特异性cDNA探针鉴定H-2Kd基因。
EMBO J. 1982;1(4):467-71. doi: 10.1002/j.1460-2075.1982.tb01192.x.
4
cDNA clone coding for part of a mouse H-2d major histocompatibility antigen.编码小鼠H-2d主要组织相容性抗原部分序列的cDNA克隆
Proc Natl Acad Sci U S A. 1981 May;78(5):2772-6. doi: 10.1073/pnas.78.5.2772.
5
Isolation of a cDNA clone for the human HLA-DR antigen alpha chain by using a synthetic oligonucleotide as a hybridization probe.使用合成寡核苷酸作为杂交探针分离人HLA - DR抗原α链的cDNA克隆。
Proc Natl Acad Sci U S A. 1982 Oct;79(19):5966-70. doi: 10.1073/pnas.79.19.5966.
6
Mouse histocompatibility genes: structure and organisation of a Kd gene.小鼠组织相容性基因：Kd基因的结构与组织
EMBO J. 1983;2(2):245-54. doi: 10.1002/j.1460-2075.1983.tb01413.x.
7
Sequence of gene and cDNA encoding murine major histocompatibility complex class II gene A beta 2.编码小鼠主要组织相容性复合体II类基因Aβ2的基因和cDNA序列。
J Biol Chem. 1985 Nov 15;260(26):14111-9.
8
Comparison of nucleotide sequences of mRNAs belonging to the mouse H-2 multigene family.属于小鼠H-2多基因家族的mRNA核苷酸序列比较。
Nucleic Acids Res. 1982 Feb 11;10(3):1039-49. doi: 10.1093/nar/10.3.1039.
9
10
Identification of an H-2Kb-related molecule by molecular cloning.通过分子克隆鉴定一种与H-2Kb相关的分子。
Immunogenetics. 1981;14(5):383-92. doi: 10.1007/BF00373318.
引用本文的文献
1
Serological and immunochemical analysis of H-2 class I molecules encoded by the Db region.对由Db区域编码的H-2 I类分子进行血清学和免疫化学分析。
Immunogenetics. 1984;20(3):341-5. doi: 10.1007/BF00364215.
2
Alternative splicing in the 5' moiety of the H-2Kd gene transcript.H-2Kd基因转录本5'部分的可变剪接。
EMBO J. 1984 Oct;3(10):2383-6. doi: 10.1002/j.1460-2075.1984.tb02143.x.
3
DNA sequence of the mouse H-2Dd transplantation antigen gene.小鼠H-2Dd移植抗原基因的DNA序列。
Proc Natl Acad Sci U S A. 1985 Feb;82(4):1175-9. doi: 10.1073/pnas.82.4.1175.
4
Two subtypes of BfF by isoelectrofocusing: differential linkage to other HLA markers.通过等电聚焦法鉴定的BfF的两种亚型：与其他HLA标记的差异连锁
Hum Genet. 1985;69(2):181-3. doi: 10.1007/BF00293294.
5
An H-2K gene of the tw32 mutant at the T/t complex is a close parent of an H-2Kq gene.位于T/t复合体的tw32突变体的H-2K基因是H-2Kq基因的近源亲本。
Immunogenetics. 1985;21(4):367-83. doi: 10.1007/BF00430802.
6
Alternative processing of H-2Dd pre-mRNAs results in membrane expression of differentially phosphorylated protein products.H-2Dd前体mRNA的可变加工导致差异磷酸化蛋白产物的膜表达。
EMBO J. 1986 Oct;5(10):2477-83. doi: 10.1002/j.1460-2075.1986.tb04524.x.
7
DQw1 beta gene from HLA-DR2-Dw12 consists of six exons and expresses multiple DQw1 beta polypeptides through alternative splicing.来自HLA - DR2 - Dw12的DQw1β基因由六个外显子组成，并通过可变剪接表达多种DQw1β多肽。
Immunogenetics. 1987;25(5):343-6. doi: 10.1007/BF00404428.
8
Alternative splicing in the mouse H-2Kd gene is not necessary for the classical Kd antigen function.小鼠H-2Kd基因中的可变剪接对于经典Kd抗原功能并非必需。
Immunogenetics. 1986;24(4):267-74. doi: 10.1007/BF00364531.
9
Qa gene expression: biosynthesis and secretion of Qa-2 molecules in activated T cells.Qa基因表达：活化T细胞中Qa - 2分子的生物合成与分泌
Proc Natl Acad Sci U S A. 1986 May;83(9):2949-53. doi: 10.1073/pnas.83.9.2949.
10
HLA-JY328: mapping studies and expression of a polymorphic HLA class I gene.HLA-JY328：一个多态性HLA I类基因的定位研究与表达
Immunogenetics. 1986;23(2):90-9. doi: 10.1007/BF00377967.
本文引用的文献
1
The DNA sequence of the H-2kb gene: evidence for gene conversion as a mechanism for the generation of polymorphism in histocompatibilty antigens.H-2kb基因的DNA序列：基因转换作为组织相容性抗原多态性产生机制的证据。
EMBO J. 1983;2(3):453-62. doi: 10.1002/j.1460-2075.1983.tb01444.x.
2
Mouse histocompatibility genes: structure and organisation of a Kd gene.小鼠组织相容性基因：Kd基因的结构与组织
EMBO J. 1983;2(2):245-54. doi: 10.1002/j.1460-2075.1983.tb01413.x.
3
Correlation of DNA exonic regions with protein structural units in haemoglobin.血红蛋白中DNA外显子区域与蛋白质结构单元的相关性。
Nature. 1981 May 7;291(5810):90-2. doi: 10.1038/291090a0.
4
A catalogue of splice junction sequences.剪接连接序列目录。
Nucleic Acids Res. 1982 Jan 22;10(2):459-72. doi: 10.1093/nar/10.2.459.
5
DNA sequence of a gene encoding a BALB/c mouse Ld transplantation antigen.编码BALB/c小鼠Ld移植抗原的基因的DNA序列。
Science. 1982 Feb 5;215(4533):679-82. doi: 10.1126/science.7058332.
6
Polymorphism of the mouse H-2 loci.小鼠H-2基因座的多态性。
Immunol Rev. 1981;60:23-57. doi: 10.1111/j.1600-065x.1981.tb00361.x.
7
Primary structural: analysis of the transplantation antigens of the murine H-2 major histocompatibility complex.主要结构：小鼠H-2主要组织相容性复合体移植抗原的分析
Annu Rev Biochem. 1981;50:1025-52. doi: 10.1146/annurev.bi.50.070181.005113.
8
The traditional and a new version of the mouse H-2 complex.传统版和新版小鼠H-2复合体。
Nature. 1981 Jun 11;291(5815):455-60. doi: 10.1038/291455a0.
9
Isolation of a cDNA clone for the murine transplantation antigen H-2Kb.小鼠移植抗原H-2Kb的cDNA克隆的分离
Proc Natl Acad Sci U S A. 1982 May;79(10):3270-4. doi: 10.1073/pnas.79.10.3270.
10
Structure and expression of a mouse major histocompatibility antigen gene, H-2Ld.小鼠主要组织相容性抗原基因H-2Ld的结构与表达
Proc Natl Acad Sci U S A. 1982 Mar;79(6):1994-8. doi: 10.1073/pnas.79.6.1994.
Zotero 插件