• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

酿酒酵母精氨酸酶基因（CAR1）的核苷酸序列及其在不同生理条件下的转录情况。

Nucleotide sequence of the Saccharomyces cerevisiae arginase gene (CAR1) and its transcription under various physiological conditions.

作者信息

Sumrada R A, Cooper T G

出版信息

J Bacteriol. 1984 Dec;160(3):1078-87. doi: 10.1128/jb.160.3.1078-1087.1984.

DOI:10.1128/jb.160.3.1078-1087.1984
PMID:6094498
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215822/
Abstract

We have determined the nucleotide sequence of the yeast CAR1 gene along with its 5' and 3' flanking sequences. The structural portion of this gene encodes a protein of 333 amino acid residues with a calculated Mr value of 35,616. The transcripts produced from this gene are modestly heterogeneous at their 5' and 3' termini. Most 5' termini map to a position 42 to 49 base pairs upstream from the ATG at the beginning of the open reading frame. The 3' termini map to a position 108 to 127 base pairs downstream of the amber codon which terminates the open reading frame. A variety of potentially significant sequences were identified in the regions flanking the structural portion of the gene.

摘要

我们已经确定了酵母CAR1基因的核苷酸序列及其5'和3'侧翼序列。该基因的结构部分编码一个由333个氨基酸残基组成的蛋白质，计算所得的Mr值为35,616。该基因产生的转录本在其5'和3'末端存在一定程度的异质性。大多数5'末端定位在开放阅读框起始处ATG上游42至49个碱基对的位置。3'末端定位在终止开放阅读框的琥珀密码子下游108至127个碱基对的位置。在该基因结构部分的侧翼区域鉴定出了多种潜在的重要序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3446/215822/0795d534c38f/jbacter00229-0262-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3446/215822/9ff9a121ff9b/jbacter00229-0260-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3446/215822/aed78a301f8b/jbacter00229-0260-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3446/215822/acab9ea74b0f/jbacter00229-0261-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3446/215822/bbaa0e948bfb/jbacter00229-0261-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3446/215822/0795d534c38f/jbacter00229-0262-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3446/215822/9ff9a121ff9b/jbacter00229-0260-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3446/215822/aed78a301f8b/jbacter00229-0260-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3446/215822/acab9ea74b0f/jbacter00229-0261-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3446/215822/bbaa0e948bfb/jbacter00229-0261-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3446/215822/0795d534c38f/jbacter00229-0262-a.jpg

相似文献

1
Nucleotide sequence of the Saccharomyces cerevisiae arginase gene (CAR1) and its transcription under various physiological conditions.酿酒酵母精氨酸酶基因（CAR1）的核苷酸序列及其在不同生理条件下的转录情况。
J Bacteriol. 1984 Dec;160(3):1078-87. doi: 10.1128/jb.160.3.1078-1087.1984.
2
Yeast regulatory gene PPR1. I. Nucleotide sequence, restriction map and codon usage.酵母调控基因PPR1。I. 核苷酸序列、限制性图谱及密码子使用情况。
J Mol Biol. 1984 Dec 5;180(2):239-50. doi: 10.1016/s0022-2836(84)80002-9.
3
Ubiquitous upstream repression sequences control activation of the inducible arginase gene in yeast.普遍存在的上游抑制序列控制酵母中诱导型精氨酸酶基因的激活。
Proc Natl Acad Sci U S A. 1987 Jun;84(12):3997-4001. doi: 10.1073/pnas.84.12.3997.
4
Multiple positive and negative cis-acting elements mediate induced arginase (CAR1) gene expression in Saccharomyces cerevisiae.多个正向和负向顺式作用元件介导酿酒酵母中诱导型精氨酸酶（CAR1）基因的表达。
Mol Cell Biol. 1990 Oct;10(10):5087-97. doi: 10.1128/mcb.10.10.5087-5097.1990.
5
Primary structure of the multifunctional alpha subunit protein of yeast fatty acid synthase derived from FAS2 gene sequence.源自FAS2基因序列的酵母脂肪酸合酶多功能α亚基蛋白的一级结构
J Biol Chem. 1988 Sep 5;263(25):12315-25.
6
The ILV5 gene of Saccharomyces cerevisiae is highly expressed.酿酒酵母的ILV5基因高度表达。
Nucleic Acids Res. 1986 Dec 22;14(24):9631-51. doi: 10.1093/nar/14.24.9631.
7
A cis-acting element present in multiple genes serves as a repressor protein binding site for the yeast CAR1 gene.存在于多个基因中的一个顺式作用元件充当酵母CAR1基因的阻遏蛋白结合位点。
Mol Cell Biol. 1990 Aug;10(8):3884-95. doi: 10.1128/mcb.10.8.3884-3895.1990.
8
The nucleotide sequence of the yeast ARG4 gene.酵母ARG4基因的核苷酸序列。
Gene. 1984 Sep;29(3):271-9. doi: 10.1016/0378-1119(84)90056-8.
9
The RAD2 gene of Saccharomyces cerevisiae: nucleotide sequence and transcript mapping.酿酒酵母的RAD2基因：核苷酸序列与转录图谱
Gene. 1985;36(3):225-34. doi: 10.1016/0378-1119(85)90177-5.
10
Nucleotide sequence, transcript mapping, and regulation of the RAD2 gene of Saccharomyces cerevisiae.酿酒酵母RAD2基因的核苷酸序列、转录图谱及调控
J Bacteriol. 1986 Jun;166(3):914-23. doi: 10.1128/jb.166.3.914-923.1986.

引用本文的文献

1
Self-protective responses to norvaline-induced stress in a leucyl-tRNA synthetase editing-deficient yeast strain.亮氨酰 - tRNA合成酶编辑缺陷型酵母菌株对正缬氨酸诱导应激的自我保护反应。
Nucleic Acids Res. 2017 Jul 7;45(12):7367-7381. doi: 10.1093/nar/gkx487.
2
Characterization of Bacillus anthracis arginase: effects of pH, temperature, and cell viability on metal preference.炭疽芽孢杆菌精氨酸酶的特性：pH、温度和细胞活力对金属偏好的影响
BMC Biochem. 2008 Jun 3;9:15. doi: 10.1186/1471-2091-9-15.
3
Isolation and characterization of the actin gene from Tetrahymena thermophila.

本文引用的文献

1
A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts.对酵母分类中常用的氮同化试验的批判性评价。
J Bacteriol. 1946 Sep;52(3):293-301.
2
Isolation of the CAR1 gene from Saccharomyces cerevisiae and analysis of its expression.从酿酒酵母中分离CAR1基因并分析其表达。
Mol Cell Biol. 1982 Dec;2(12):1514-23. doi: 10.1128/mcb.2.12.1514-1523.1982.
3
The promoter region of the arg3 gene in Saccharomyces cerevisiae: nucleotide sequence and regulation in an arg3-lacZ gene fusion.
从嗜热四膜虫中分离和鉴定肌动蛋白基因。
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5160-4. doi: 10.1073/pnas.83.14.5160.
4
Disruption of the CAR1 gene encoding arginase enhances freeze tolerance of the commercial baker's yeast Saccharomyces cerevisiae.编码精氨酸酶的CAR1基因的破坏增强了商业面包酵母酿酒酵母的耐冻性。
Appl Environ Microbiol. 2003 Jan;69(1):715-8. doi: 10.1128/AEM.69.1.715-718.2003.
5
Nitrogen catabolite repression in Saccharomyces cerevisiae.酿酒酵母中的氮代谢物阻遏
Mol Biotechnol. 1999 Aug;12(1):35-73. doi: 10.1385/MB:12:1:35.
6
G1n3p is capable of binding to UAS(NTR) elements and activating transcription in Saccharomyces cerevisiae.G1n3p能够与酿酒酵母中的UAS（NTR）元件结合并激活转录。
J Bacteriol. 1996 Jun;178(12):3470-9. doi: 10.1128/jb.178.12.3470-3479.1996.
7
On the evolution of arginases and related enzymes.关于精氨酸酶及相关酶的进化
J Mol Evol. 1994 Jul;39(1):101-4. doi: 10.1007/BF00178255.
8
Identification, cloning, sequencing, and overexpression of the gene encoding proclavaminate amidino hydrolase and characterization of protein function in clavulanic acid biosynthesis.编码前克拉维酸脒水解酶基因的鉴定、克隆、测序、过表达及在克拉维酸生物合成中蛋白质功能的表征
J Bacteriol. 1995 Jul;177(13):3714-20. doi: 10.1128/jb.177.13.3714-3720.1995.
9
General amino acid control and specific arginine repression in Saccharomyces cerevisiae: physical study of the bifunctional regulatory region of the ARG3 gene.酿酒酵母中的一般氨基酸调控和特定精氨酸阻遏：ARG3基因双功能调控区域的物理研究
Mol Cell Biol. 1985 Nov;5(11):3139-48. doi: 10.1128/mcb.5.11.3139-3148.1985.
10
Molecular cloning and nucleotide sequence of cDNA for human liver arginase.人肝脏精氨酸酶cDNA的分子克隆及核苷酸序列
Proc Natl Acad Sci U S A. 1987 Jan;84(2):412-5. doi: 10.1073/pnas.84.2.412.
酿酒酵母中arg3基因的启动子区域：arg3 - lacZ基因融合体中的核苷酸序列及调控
EMBO J. 1983;2(2):205-12. doi: 10.1002/j.1460-2075.1983.tb01406.x.
4
Transcription of adenovirus type 2 genes in a cell-free system: apparent heterogeneity of initiation at some promoters.2型腺病毒基因在无细胞体系中的转录：某些启动子起始位点的明显异质性
Mol Cell Biol. 1981 Jul;1(7):635-51. doi: 10.1128/mcb.1.7.635-651.1981.
5
Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.侧翼核苷酸在真核生物核糖体识别AUG起始密码子中的可能作用。
Nucleic Acids Res. 1981 Oct 24;9(20):5233-52. doi: 10.1093/nar/9.20.5233.
6
A simple method for displaying the hydropathic character of a protein.一种展示蛋白质亲水性特征的简单方法。
J Mol Biol. 1982 May 5;157(1):105-32. doi: 10.1016/0022-2836(82)90515-0.
7
The nucleotide sequence of the HIS4 region of yeast.酵母HIS4区域的核苷酸序列。
Gene. 1982 Apr;18(1):47-59. doi: 10.1016/0378-1119(82)90055-5.
8
Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.两个具有不同5'端且差异调控的mRNA编码酵母转化酶的细胞内形式和分泌形式。
Cell. 1982 Jan;28(1):145-54. doi: 10.1016/0092-8674(82)90384-1.
9
Physical analysis of mating-type loci in Saccharomyces cerevisiae.酿酒酵母交配型位点的物理分析。
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:961-81. doi: 10.1101/sqb.1981.045.01.113.
10
Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo.通过对体内特定缺失突变体的研究鉴定H2A组蛋白基因前奏序列中的调控序列。
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1432-6. doi: 10.1073/pnas.77.3.1432.